ISOLATION OF SECONDARY METABOLITES FROM Fusarium solani, AN ENDOPHYTIC FUNGUS OF Cryptocarya pulchrinervia

• Main Author: Amelia Sari, Santi
• Format: Theses
• Language: Indonesia
• Subjects: Kimia
• Online Access: https://digilib.itb.ac.id/gdl/view/35843
• Institution: Institut Teknologi Bandung

Endophytic fungi are microorganisms that can be used as an alternative source of bioactive compounds. Endophytic fungi are found in various plant tissues without causing negative effect to the host plant. Taxol is one of the important secondary metabolites that has been reported from endophytic fungi. This compound has been approved by food and drug administration (FDA) as cancer treatment. Taxol was isolated for the first time from Taxus brevifolia. Recently biotechnology of plants showed that taxol can also be isolated from endophytes Taxus brevifolia. One of the potential Indonesia plants as the host for endophyte is Cryprocarya in family Lauraceae. Besides being used as traditional medicine, this genus has been reported to produce secondary metabolites including flavonoids, ?-pyrons and alkaloids with various bioactivity. However, phytochemical studies of endophyte from Cryprocarya are still limited, where only one spesies of endophytic fungus was reported from Cryptocarya. Based on recent literatur, chemical evaluation of secondary metabolites isolated from Indonesian Cryptocarya has not been reported. In this research, isolation and characterization of secondary metabolites from endophytes isolated from Crytocarya pulchrinervia followed by evaluation of cytotoxicity against murine leukemia P388 cells were carried out. Two single strains, endophytic fungi CP1 and CP2, were isolated from twigs of C. pulchrinervia. Fungus of CP2 was identified as Fusarium solani in microbology laboratory LIPI Cibinong based on genetic analyze Internal Transcribed Spacer (ITS) DNA. F. solani was cultivated into 10 L of Potato Dextrose Broth (PDB) (24 g/1 L H2O) medium at 28 oC for two weeks. 7.0 gram methanol extract from mycelial and 1.2 gram EtOAc extract from liquid medium were obtaned from this fungus. Methanol extract and EtOAc extract were separated using various chromatography methods such as vacuum liquid chromatography and column chromatography to obtain eight pure compounds. All compounds were characterized based on spectroscopic data including NMR 1D (1H and 13C) and 2D (HSQC dan HMBC). Three pure compounds were isolated from methanol extract namely (22E,24R)-ergosta-5,7,22-dien-3?-ol (1), 5?,8?-epidioxy-(22E,24R)- ergosta-6,22-dien-3?-ol (2) and oleic acid (3). Other compounds were isolated from EtOAc extract identified as tyrosol (4), javanicin (5), fusarubin (6), 3-O- methylfusarubin (7), and dihydronaphthalenone (8). Evaluation of compounds (1- 8) for cytotoxic activity against murine leukemia P388 cell showed that compound (5) and (6) were showed very active with IC50 0.95 µg/mL and 3.93 µg/mL, respectively. While compounds (1), (2), (4), (7) and (8) have IC50 4.45 µg/mL; 4.62 µg/mL; 5.84 µg/mL; 21.21 µg/mL; 42.50 µg/mL. It showed these compounds not active.