Resistance status of some strains of house flies Musca domestica (Diptera: Muscidae) to permethrin insecticide and propoxur as well as synergistic butoxide piperonyl effects

• Main Author: Susanti, Silvi
• Format: Theses
• Language: Indonesia
• Subjects: Ilmu hayati ; Biologi
• Online Access: https://digilib.itb.ac.id/gdl/view/36009
• Institution: Institut Teknologi Bandung

House fly Musca dometica is one urban pest that causes harm to humans, poultry in terms of health and in term aesthetics. Common procedure that use to control its population by using insecticides. However, unwise use of insecticides can lead to resistance. In some countries has been reported on the status of house fly resistance.Unfortunately, there has been no report on the status of house fly resistance to insecticides in Indonesia especially on pyrethroid (permethrin 94%) and carbamate (propoxur 99.25%) that commonly use in Indonesia. Thus, test is conducted on the house fly strains JKT, BDG, and SBY YGY. The method used in this study is topical application method. The observed variable is the mortality rate of flies that were analyzed using POLO PC to determine the value of LD50 and LD90 (Lethal Dose). Results then use to determine the value of RR50 and RR90 (Resistance Ratio) which state the status of resistance to insecticides. On the other hand to observe the resistance mechanisms, I applied the insecticide solution that mixed with PBO (piperonil butoxide) (ratio of 1:5; insecticide: PBO). PBO as a synergistic work that can inhibit enzymes MFO (Mixed Function Oxidase) which is shown by value of SR (Synergist Ratio). Resistance tests showed that the strain BDG has became resistant strains to permethrin with LD90 228.7 x 10-3 ?g/fly and RR90 133.7X while the most susceptible strain was YGY with the LD90 1.71 x 10-3. Strain SBY with LD90 62 771 x 10-3 ?g/fly and RR90 38.36X was the most resistant strain to propoxur, followed by BDG with LD90 29 821 x 10-3 ?g/fly, RR90 18.22X, and the most vulnerable is strain YGY with LD90 1636,27 x 10-3 ?g/fly. After the addition of PBO, all LD50 value for permethrin YGY generally decreases with SR 1.35, BDG with SR 2.23, and SBY with SR 3.59. As for propoxur BDG with SR 4.07, JKT with SR 11.08 and SBY with SR 0. Based on this data, I conclude that the strain of BDG has experienced resistance to permethrin and propoxur, SBY only experiencing strains resistant to propoxur alone, and strains YGY is still susceptible strains among the four strains of both types of insecticides. Addition of PBO lowered the value of LD and indicating MFO play important role in the occurrence of resistance in resistant flies, although presumably other mechanisms also work together.