POLYPHENOL EFFECT TO INSULIN RECEPTOR-? EXPRESSION ON THE SCELETAL MUSCLE OF DIABETIC-INDUCED MICE

• Main Author: Riany, Hesti
• Format: Theses
• Language: Indonesia
• Subjects: Ilmu hayati ; Biologi
• Online Access: https://digilib.itb.ac.id/gdl/view/36038
• Institution: Institut Teknologi Bandung

Diabetes Mellitus is a metabolic disorder caused by abnormal insulin secretion that is characterized by hyperglycemia. Hyperglycemia can increase reactive oxygen species (ROS) production in the cell. Elevated ROS levels may also contribute to accelerate diabetes onset or diabetes complication by some defects in insulin and insulin receptor binding or signaling. Insulin receptor-? (IR?) is a transmembrane receptor that form a part (sub unit) of insulin receptor. Insulin and insulin receptor binding will influence translocation of GLUT 4 into cell surface that it has contribution in glucose up take. Polyphenol from green tea (Polyphenol) is an antioxidant that has effect to decrease ROS level. In the previous study was known that consumption of polyphenol by diabetic mice enhanced translocation GLUT 4 into plasma membrane, although it couldn’t decrease blood glucose level into normal condition. In the signaling insulin process, GLUT 4 translocation have concerned with IR? expression. Hence, the aim of this study is to understand how polyphenol could affect to the IR? expression due to repair glucose up take in diabetic mice. In this study, mice (ddY) were induced become diabetic by intra venous injection of Alloxan doses 70 mg/kg body weight. Diabetic mice with blood glucose ? 200 mg/dl were used. Mice were divided into three groups, there were normal group, diabetic mice group that were given control solution know as (P0) group and diabetic mice that were given 100 mg/kg body weight of polyphenol (P100) group. Each animal were given control solution and polyphenol daily by using an intragastric tube for 22 days. Measurement of blood glucose level administered every 3 days in the fasting state. In the end of treatment, all animals were subjected to a glucose tolerance test and skeletal muscle tissue from all mouse were isolated. IR? proteins were isolated from muscle tissue of forelimb and hindlimb region and then the expression were observed by using Western Blotting method (WB). Blotted proteins were examined by using enhanced chemiluminescene (ECL) and were quantified by scion image program. iii iv Results of this study shown that blood glucose of P0 group (325 mg/dl) is higher than P100 group (266 mg/dl) and normal group (101 mg/dl) and also after 2 hour post prandial (PP), blood glucose concentration of P0 group (273 mg/dl) higher than P100 group (268 mg/dl) and normal group (106 mg/dl). IR? expression from all group (normal, P0, P100) based on condition (fasting, 1 hour PP and 2 hour PP) have shown two different pattern of expression. First, in 1 hour PP indicate a tendency that IR? expression in diabetic mice is lower than normal mice and IR? expression of P100 group is the lowest. Second, in the fasting and 2 hour PP indicated a tendency that IR? expressions in diabetic mice (P0 and P100 groups) is higher than normal mice and IR? expression of P100 group is the highest. In 1 hour PP has been indicated that polyphenol lead the IR? internalization process whereas in 2 hour PP has been indicated that polyphenol lead the IR? trafficking into plasma membrane. Based on the results of this study can be concluded that polyphenol might be increase IR? expression in diabetic mice although polyphenol administered couldn’t decrease blood glucose concentration into normal condition.