EFFECT MUNICIPAL SLUDGE WASTE LEACHATE FROM TPA SARIMUKTI ON MICE PREIMPLANTATION EMBRYONIC DEVELOPMENT (Mus musculus L.) SW

• Main Author: Agnhitya Sari, Tantry
• Format: Theses
• Language: Indonesia
• Subjects: Ilmu hayati ; Biologi
• Online Access: https://digilib.itb.ac.id/gdl/view/36055
• Institution: Institut Teknologi Bandung

TPAs Sarimukti is a final disposal site for municipal waste from Bandung, Cimahi and West Bandung regency. Waste management at TPAs Sarimukti is nonsanitary landfill which can produces a sludge waste. Results of previous studies showed that the leachate of Sarimukti TPAs contain many compounds such as organic, inorganic and heavy metals (Garnasih & Yusuf, 2009; Yusuf & Kurniawan, 2010). Pregnant women in the vicinity of TPAs can be exposed by the sludge leachate so it does not close the possibility of individuals develop in the womb are also exposed to the compounds contained in the sludge leachate. Individuals who are developing especially in early development, when exposed by a toxic material can cause various problems such as delay of development, malformations and early spontaneous abortion (Nagao et al., 2006). The aim of this research is to evaluate the municipal sludge waste leachate from TPAs Sarimukti to the mouse preimplantation embryonic development. Female mice (810 week-old, 30-37 g) were superovulated with 5 IU of PMSG (Sigma) by intraperitoneal injection and after 48 hour mice were then injected with 5 IU of hCG (Chorulon) by the same way. After this second injection, female mice were mated with male mice overnight. A vaginal plug detected as the following morning was confirmed as day 0 of gestation. The mice then divided in to three groups (leachate-treated group, negative control group, positive control group). Leachate-treated mice group injected with three range municipal sludge waste leachate concentrations (17%, 29%, 50%), negative control mice injected with aquabidestilata sterile, while positive control mice injected with acrylamide (50 mg/kg bw). Municipal sludge waste leachate, aquabidestilata sterile and acrylamide were given by intraperitoneal injection until 3 days of gestation. At 3.5 days of gestation, mice were sacrificed by cervical dislocation and their uterus were removed. The embryos were harvested by flushing both of uterine horn then embryos were examined under a microscope. Hatched blastocysts, expanded blastocysts were classified as normal embryos. Early blastocysts, uncompacted morulae, compacted morula, cleavage stages (2-8 cell) were classified as delays embryo development. Degenerated embryos and/or unfertilized eggs, and abnormal degenerating blastocysts were classified as malformation embryos. Abnormal embryos consisted of an embryo that is experiencing a delay embryos development and malformations. Based on a statistical analysis, the percentage of abnormal embryos in the sludge leachate groups did not differ significantly (p <0.05) compared to negative control group. The percentage of abnormal embryos in the group of waste sludge leachate concentration of 17%, 29% and 50% tended to increase. The percentage of abnormal embryos waste sludge leachate group does not exceed the percentage of abnormal embryos acrylamide groups. This shows that the level of damage caused by embryo preimplantation leachate sludge is not as severe as damage caused by embryo preimplantation treated with acrylamide. Provision of waste sludge leachate with a concentration of 17%, 29% and 50% tended to increases the embryonic malformations. The type of malformation of the embryos commonly found are the degenerate embryos. The results of this study indicated that municipal sludge waste leachate from TPA Sarimukti potentially interfere with mice preimplantation embryonic development.