CYTOTOXIC ACTIVITY AND APOPTOSIS INDUCTION OF Sargassum myriocystum, Turbinaria ornata dan T. decurrens (BROWN ALGAE /PHAEOPHYTA ON BREAST CANCER CELL LINE
Breast cancer is a disease with a high prevalence. The American Cancer Society estimates that in 2015 there will be 231,840 new cases of invasive breast cancer and 60, 290 new cases of non-invasive breast cancer. Alternative therapy has been used in cancer is natural product therapy, including macro...
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| Format: | Dissertations |
| Language: | Indonesia |
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| Online Access: | https://digilib.itb.ac.id/gdl/view/36159 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Breast cancer is a disease with a high prevalence. The American Cancer Society estimates that in 2015 there will be 231,840 new cases of invasive breast cancer and 60, 290 new cases of non-invasive breast cancer. Alternative therapy has been used in cancer is natural product therapy, including macroalgae (brown algae). Brown algae contain bioactive compounds that have the potential to be developed as anticancer. However, active compounds from semi polar and polar extracts of Indonesian brown algae have not been widely studied, so it needs to prove their anticancer activity in cell line.
The aims of this study are to screen brown algae species that had potential anticancer activities and determine the effect of the bioactive compounds on proliferation and apoptosis of breast cancer cell lines T47D and MCF-7. The study was conducted in 2 stages: Stage 1: the selection of extracts from various species of brown algae with Brine shrimp lethality test (BSLT) assay using Artemia salina larvae. Stage 2: fractionation from algae extract using vacuum liquid chromatography (KVC) to separate bioactive compounds in the extract. Fractions were tested in breast cancer cell lines T47D and MCF-7 to know their cytotoxic activity and apoptosis induction. Cytotoxic activity was tested by using MTT assay and detection of apoptosis was evaluated apoptosis by flowcytometry (Annexin-PI staining).
Five species of brown algae that collected from Santolo Beach, Pameungpeuk, Garut i.e: Sargassum sp., S. duplicatum, S. myriocystum, T. ornata and T. decurrens (identified by Research Center for Oceanography LIPI) are washed, dried, milled and extracted with n-hexane and ethyl acetate. The toxicity of each extract was tested with Brine Shrimp Lethality Test (BSLT). Three species of brown algae that had the lowest LC50 are T. ornata (103,3 ?g / ml), T. decurrens
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(216.12 ?g / ml) and S. myriocystum (275, 28 ?g / ml) were selected for further testing. Phytochemical screening result is known that compounds contained in the extract are terpenoids.
Results of second stage are obtained 15 fractions of a third extract selected species (T. ornata, T. decurrens and S. myriocystum) which were then tested in breast cancer cell lines T47D and MCF-7. Results of cytotoxicity assay showed that the lowest IC50 were the fraction 7 (T. ornata; 86, 763 ?g / ml), the fraction 4 (Turbinaria decurrens; 33.713 ?g / ml and fraction 6 (S. myriocystum; 47 578 ?g / ml) in T47D cells and fraction 4 (T. ornata; 86.768 ?g / ml), the fraction 6 (T. decurrens; 92 099 ?g / ml and fraction 5 (S. myriocystum; 133, 76 ?g/ ml) in MCF-7 cells. Fraction 7 (T. ornata; 86, 763 ?g /ml ) was able to induce apoptosis by the percentage of cells undergoing apoptosis at 78, 37 %. This showed that the fraction TO7 (T. ornata) could inhibit cell proliferation and induced apoptosis. TO7 contain triacontane that may play in induce apoptosis |
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