THE PROTECTIVE EFFECT OF PROPOLIS NANOEMULSION (NEP) AGAINST UVB IRRADIATION INDUCED PHOTOAGING IN HUMAN DERMAL FIBROBLAST (HDF)

• Main Author: Agustin, Rini
• Format: Theses
• Language: Indonesia
• Subjects: Ilmu hayati ; Biologi
• Online Access: https://digilib.itb.ac.id/gdl/view/36325
• Institution: Institut Teknologi Bandung

Aging (aging) is a physiological process that can occur in all organs of the body including the skin. Exposure of the skin to ultraviolet B (UVB) radiation causes oxidative damage to skin, resulting in photoaging. It is generally believed that the skin damage induced by UV irradiation is a consequence of generation of reactive oxygen species (ROS). Reactive oxygen species can increase the rate of skin aging, hereinafter referred to as premature skin aging due to UV radiation (photoaging). Currently aging has been considered as a disease that needs to be undertaken to prevent and cure, one of them with the use of antioxidants. Indonesia as a country with high biodiversity, potentially resulting in natural materials as a source of antioxidant that can inhibit the process of photoaging. Some natural substances derived from biodiversity in Indonesia has been widely studied has good potential as an antioxidant and photoprotective agent. Propolis is one of the richest natural product sources of polyphenols, derived from various plant resins collected by honeybees, and has been used as a folk medicine, natural cosmetics and food supplements for centuries. Availability of propolis in nature is very limited, hence the need for efficient use of propolis with the development nanoemulsi propolis (NEP). This study aimed to determine the due administration of the NEP as an anti-photoaging by observing the concentration of intracellular ROS and lipid peroxides in human dermal fibroblast cells (HDF) by exposure to UVB. Bioactive compound in propolis was analysed by GC-MS, the NEP-making was done by sonication and coating. The analysis and determination of the optimum concentration of cytotoxic NEP conducted by MTT assay, analysis of intracellular ROS concentration measurement by using DCFDA assay, measurement of lipid peroxide products by using the TBARS assay and morphological observation of the cell nuclei condition by using DAPI staining was observed using a confocal microscope. PSA analysis shown the size of NEP around 131.9 nm 131 nm successfully made with a composition of 30% kolliphor RH40, 10% glycerol, 5% VCO (virgin coconut oil) and 3% EEP (ethanol extract of propolis). The highest cell viability was shown in 0,1?g / ml given in HDF cells for 1hr of NEP concentration and considered as an optimum concentration. The results of measurement of the ROS product in cells HDF with UVB exposure 200mJ / cm2 (3.474 au) showed increased ROS significantly (P <0.05) to approximately twice the normal state (1.928 au), while the treatment of NEP (2.855 au) ROS product showed significant decrease (P <0.05). Observations measurement of lipid peroxide products showed the same pattern as the result of the measurement of concentration of ROS. The results of the measurement of lipid peroxide concentration in the fibroblasts with UVB exposure 200mJ / cm2 (0.488 nmol / mg protein) showed an increase in lipid peroxide significantly (P <0.05) to almost close to three times the normal state (0.168 nmol / mg protein), while the treatment of NEP (0.403 nmol / mg protein) showed a decrease of lipid peroxide concentrations significantly (P <0.05). As a conclusion based on these results that the propolis in NEP acts as an antioxidant and has the potential to reduce the production of ROS and lipid peroxides caused by exposure to UVB.