EXPRESSION STUDY OF MaACO AND MaACS2 GENES IN SUSCEPTIBLE AND RESISTANT BANANA TO BLOOD DISEASE
Indonesia is one of the tropical fruits producer countries such as banana. Genetic diversity of banana is very high in Indonesia (more than 200 have been identified from more than ~1000). However, banana genetic information remains limited, particularly related to disease resistance. Blood Diseas...
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| Format: | Theses |
| Language: | Indonesia |
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| Online Access: | https://digilib.itb.ac.id/gdl/view/36637 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Indonesia is one of the tropical fruits producer countries such as banana. Genetic
diversity of banana is very high in Indonesia (more than 200 have been identified
from more than ~1000). However, banana genetic information remains limited,
particularly related to disease resistance. Blood Disease is one of the common
disease caused by Blood Disease Bacterium (BDB) which cause huge loss of
banana production in Indonesia. Gene family of MaACO and MaACS2 play role
in ethylene biosynthesis which is responsible for respon of plant defense
mechanism. However, studies regarding expression of such genes on plant
defense mechanism on blood disease are not available. This study aimed to
observe effect of infection Blood Disease Bacterium (BDB) on MaACO and
MaACS2 expression of susceptible and resistant banana to blood disease. BDB
isolates were identified from blood disease-infected Nangka banana (AAB group)
in Balai Penelitian Buah Tropika, Subang. BDB isolate was 99%-100%
homologous to BDB by PCR with a pair of Ralsonia solanacearum (RS) primer
and a pair of BDB spesific primer. In this study, there were two banana cultivars
observed, Mas Kirana (AA group) as a resistant candidate to blood disease, and
Klutuk (BB group) as a susceptible candidate to blood disease. BDB injection in
suspension of 2,5 ml BDB with density of 107 cfu/ml, was conducted in 6 months
old banana on 5 cm above ground surface. Symptoms development was observed
in injected banana. Gene expression of MaACO and MaACS2 was measured with
Reverse Transcriptase (RT) PCR. Results showed that blood disease symptoms
were present in BDB-injected banana in Klutuk group after 1 week, which were
shown as flaccid, withered leaf, yellowish, a reddish-brown discoloration of stem,
corm turned brown and rot, whereas there was no symptoms found significantly in
Mas Kirana group as a resistant candidate to BDB. Molecular analysis showed
MaACO and MaACS2 were not significantly in gene expression both in Mas
Kirana and Klutuk after BDB injection. |
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