PROCESS OPTIMIZATION AND CHARACTERIZATION OF NANOPARTICLE ANTISENSE OLIGONUCLEOTIDES EBA175 AND DHS TARGETED
The development of new antimalarial drugs is important for eliminating malaria. One of the strategies expected is the development of antisense oligonucleotides (ASOs) therapy which can be used specifically and targeted as a protein sinthesis inhibitor of P. falciparum such as EBA175 and DHS prote...
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| Format: | Theses |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/36978 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | The development of new antimalarial drugs is important for eliminating malaria. One
of the strategies expected is the development of antisense oligonucleotides (ASOs)
therapy which can be used specifically and targeted as a protein sinthesis inhibitor of
P. falciparum such as EBA175 and DHS protein. To reach the mRNA target, ASOs
need to be delivered by a delivery system that can protect enzymatic degradation and
increase its permeability. This research formulated and characterized nanoparticles
(NP) ASOs as a system used to deliver ASOs. The NP system used was cationic
chitosan polymer with a combination of PLGA and poloxamer. The NP was prepared
by precipitation method in which stirring rate and stirring time were optimized. From
the experiments carried out, ASO NPs with particle size characteristics <200 nm,
polydispersion index <0.5, positive zeta potential, and spherical morphology were
produced from a NP formula prepared at stirring rate 900 rpm for 60
minutes.Antimalarial activities was performed by enzymatic LDH assay method. From
the tests conducted, ASO NP showed inhibitory activity on the growth of P. falciparum.
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