AGARWOOD FORMATION BY Gyrinops versteegii (Gilg.) Domke IN RESPONSE TO Fusarium solani INOCULATION

• Main Author: Ganiyasa Susanto, Galih
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/37014
• Institution: Institut Teknologi Bandung

Agarwood is an accumulation of resin consisting of fragrant secondary metabolites secreted as a self-defense response to wounds, pathogens, and other stressful effects. Among agarwood-producing species, Gyrinops versteegii has not been widely used. This study aims to determine agarwood formation in G. versteegii as a response to the injection and inoculation of Gorontalo (GSL1) and Jambi (GSL2) strain Fusarium solani, and Potato Dextrose Broth (PDB) medium for 3 months. The responses observed include the area of discoloration as well as analysis through an olfactory test, anatomical observations, and metabolite detection using GC-MS. The results showed that the injected G. versteegii trees responded differently compared to the control trees. The response was characterized by the formation of discoloration zone around the injection area due to the inoculation of F. solani that is greater in the GSL1 strain, followed by the GSL2 strain and the PDB medium, respectively. Histological observations showed the formation of included phloem which is a structure for storing agarwood resin in the GSL1, GSL2, and PDB medium treatments. The olfactory test results showed that all agarwood samples of GSL1 and GSL2 inoculations are more fragrant than the agarwood samples injected with the PDB medium, however samples from all three treatments are more fragrant than the control samples. GC-MS analysis detected several compounds commonly found in agarwood such as 1-Octadecene; Benzene, 1,3-bis (1,1-dimethylethyl) -; 2,4-Di-tert-butylphenol; and Hexadecanoic acid, methyl ester with the sample from the GSL1 treatment having the highest concentrations. Based on the formation of the discoloration zone, aroma, and content of secondary metabolites, it can be concluded that the inoculation of F. solani GSL1 strain is best for inducing agarwood formation in G. versteegii.