COMPARISON OF ENDOPHYTIC FUNGI DIVERSITY AND CARBOHYDRATE CONTENT OF CILEMBU SWEET POTATO (Ipomoea batatas) RANCING VARIETY WERE PLANTED IN THE DIFFERENT PLACE DURING STORAGE

• Main Author: Novia Tresnaati, Wulan
• Format: Theses
• Language: Indonesia
• Subjects: Ilmu hayati ; Biologi
• Online Access: https://digilib.itb.ac.id/gdl/view/37070
• Institution: Institut Teknologi Bandung

Cilembu is one special regions that can product sweetpotato different from sweetpotato that grow in other areas. Baked Cilembu sweetpotato generated sticky sugar like honey with sweet taste. In this research, endophytic fungi was isolated, then isolates were screened for ability to produce extracellular amylase and carbohydrate (starch, glucose, and fructose) was analysed from cilembu sweetpotato same variety rancing, but planted in two place with different composition of the soil and environmental conditions. Cilembu sweetpotato was planted ini Cilembu and Cileles Jatinangor. In addition, analysis of Physico-chemical properties of the soil of the planting site is carried out in laboratories BALITSA. Carbohydrate content and endophytic fungi was analysed every week during storage period, from right after harvest to the 5th week storage time. Endophyte fungi was isolated with culturable method on PDA medium, then isolates tested for their ability to produce the enzyme amylase with selective medium YPA with 1% starch then grown colonies dropped with iodine. The starch analysis is done by converting the starch content into glucose chemically, using 25% HCL, then the starch content calculated by converting glucose levels were obtained multiplied by 0.9. Analysis of blood sugar (glucose and fructose) were performed using an HPLC column HPX87H Aminex. Total 47 endophytic fungi obtained from sweetpotato grown in Cilembu and 55 endophytic fungi obtained from sweetpotato grown in Cileles during 5 weeks of storage time. H '(Index Shannon-Wiener) of both fungal endophyte obtained from Cilembu and Cileles have includes in the medium category and C value (Index Simpson) close to 0 means that the fungal community in plant tissue is stable or no fungus dominate. There are 11 endophytic fungi from sweetpotato planted in Cilembu and 12 endophytic fungi from sweetpotato planted in Cileles Jatinangor which has the capability to produce of extracellular amylase. In addition, the average percentage of extracellular amylase endophytic fungi obtained from Cilembu iv sweetpotato is higher (11.8%) than the percentage of extracellular amylase endophytic fungi from Cileles sweetpotato (9.45%). However, percentage of extracellular amylase of both endophytic fungi were not significantly different. Carbohydrate analysis showed the starch content of both sweetpotatoes has decreased each week and starch content of sweetpotato planted in Cileles significantly higher than sweetpotato planted in Cilembu. Sweet potatoes are planted in the Cileles containing 36.97% starch just after the harvest and decreased to 33.6% at 5th week storage. Sweet potatoes are planted in the Cilembu containing 33.52% starch just after the harvest and decreased to 29.55% at 5th week storage time. In contrast, levels of sugar (glucose and fructose) from both potato has increased from just after harvest until week 4th week storage and decreased in week 5 stroge time. In addition, the sugar content of sweet potato grown in Cilembu higher (Glu: 0.04 to 0.39%; Fru: 0.03 to 0.26%) compared to the sweet potatoes grown in the Cileles (Glu: 0.03 to 0.24%; Fru: 0.03 to 0.17%). However, differences in both sugar statistically not significant.