ELICITATION OF SECONDARY METABOLITES IN AGARWOOD CALLI (AQUILARIA MALACCENSIS LAMK.) BY FUSARIUM SOLANI MYCELIUM EXTRACT AND METHYL JASMONATE

• Main Author: Puji Utami, Nadia
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/37127
• Institution: Institut Teknologi Bandung

Aquilaria malaccensis is a common agarwood-producing tree cultivated in Indonesia. The agarwood can be induced by biological agents such as Fusarium solani and chemical ones, such as methyl jasmonate (MeJA). In vitro culture using A. malaccensis calli and F. solani Gorontalo strain (GSL1) ,Jambi strain (GSL2), and methyl jasmonate, as inducer secondary metabolite production, can be used as an alternative methode to study the formation of agarwood compounds. Thus, this study aims to observe the effect of elicitation by F. solani GSL1 and GSL2 strains, and methyl jasmonate on the growth and secondary metabolite production in A. malaccensis calli. The study began by conducting calli propagation on Murashige-Skoog (MS) medium with the addition of 2 ppm 2,4-D (2,4-Dichlorophenoxyacetic acid) and 0,1 ppm (6-Benzylaminopurine) BAP. The calli was subcultured to another medium containing extract from GSL1 8 mg /L, GSL2 8 mg/L, and MeJA 100 ?M, then incubated for 20 days in dark and room temperature. The calli were measured to determine growth rate and cell viability, and subjected to testing by GC-MS to determine the profile of secondary metabolites present. The results showed that the treatments with GSL1, GSL2, and MeJA did not significantly influence calli growth rate, but decreased cell viability in the GSL1 (24,12%), GSL2 (17,04%), and MeJA (33,21%) treatments. The results of the GC-MS analysis showed that typical agarwood compounds, chromone (6-methoxy-2- (4-methoxyphenetyl) -4H-chromen-4-on), were detected in the calli of the GSL2 (5,72%) and MeJA (11,16%) treatments. From the study of this study it can be concluded that MeJA is the best elicitor in inducing agarwood compounds in A. malaccensis calli.