ISOLATION AND OPTIMIZATION OF FLAVONOID EXTRACTION FROM COSMOS CAUDATUS LEAVES

• Main Author: Nofriani, Annisa
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/37132
• Institution: Institut Teknologi Bandung

Background and objectives: Cosmos caudatus is often consumed as a vegetable in Central Java or East Java-Indonesia and traditionally used to reduce high blood pressure and appetite enhancer. One of the beneficial compounds of C. caudatus leaves is flavonoid group. The research aims to isolate flavonoid compound from C. caudatus leaves and to optimize extraction process to get the highest flavonoid content from C. caudatus water leaves extract. Methods: Extraction of fresh bud was performed by boiled with water. In flavonoid isolation, water extract was continued by fractionation and subfractionation, until an isolate was obtained. Monitoring was carried out by Thin Layer Chromatography (TLC) using citroboric as spray reagent. Then the compound was purified and purity test performed by single development TLC using thee different polarities mobile phases. Optimization of extraction process was done in various temperature and time, then flavonoid content was analyzed by High Performance Liquid Chromatography (HPLC). Results: Isolate which was isolated gave yellow color after spraying with citroboric spray reagent under UV ?366 nm, and showed maximum wavelength at 262 nm and 357 nm. C. caudatus leaves which was boiled with water at 90 0 C for 40 min showed the highest flavonoid content. Conclusions: Isolated flavonoid compound was flavonol with free OH in C3. Optimum extraction to get the highest flavonoid content from water leaves extract of C. caudatus was boiled with water at 90 0 C for 40 min.