SYNTHESIS AND CHARACTERIZATION OF MOLECULARLY IMPRINTED POLYMER (MIP) EPHEDRINE FOR EPHEDRINE SEPARATION USING GAS CHROMATOGRAPHY

• Main Author: Nurfadhila, Lina
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/37235
• Institution: Institut Teknologi Bandung

Molecularly Imprinted Polymer (MIP) is a polymer made by molecular imprinting techniques with a good affinity to the template molecule. MIP are robust molecular recognition elements able to mimic natural recognition entities, such as antibodies and biological receptors, useful to separate and analyze complicated samples such as urine. Urine may cause interferes in Gas Chromatography (GC) analysis, therefore samplepreparation methods with high selectivity is required. Ephedrine belongs to the stimulant category of doping in the World Anti-Doping Agency (WADA) list. The stimulant effect of ephedrine potentially misused to improve athletic performance by athletes. However, the medical use of ephedrine at the therapeutic level is tolerated by WADA. Urine concentrations of more than 10 mg/mL are considered as doping. The purpose of this study was to synthesize selective Molecularly Imprinted Polymer (MIP) for separation ephedrine in urine and validation of the ephedrine analysis method using GC. Synthesis of MIP was prepared by using ephedrine as the molecular template, Metacrylic Acid (MAA) as the functional monomer, ethylenglycolol dimethacrylate (EGDMA) as a crosslinker, and 2.2'-azobisisobutironitrile (AIBN) as the initiator and dichloromethane as the porogenic solvent. Imprinting Factor (IF) is calculated by comparing the area under the curve of ephedrine on the imprinted polymer with a comparable non-imprinted polymer (NIP). MIP synthesis using bulk polymerization with a ratio of template molecules, functional monomers, cross-linker (1: 4: 20) is resulted an IF of 2,129. MIP was characterized by Fourier Transform Infrared (FTIR) and evaluated using equilibrium batch rebinding method. Selectivity test showed that MIP can be used for selective extraction of ephedrine. The recovery for urine sample which was spiked with ephedrine was 81,178% after pretreatment with Molecularly Imprinted Solid Phase Extraction (MISPE). Analysis method of ephedrine with GC gave valid result, linearity with r2 = 0,9993, accuracy with recovery of 99,85 –100,78%, and precision with coefficient of variance 0,997%, detection limit of 3,001 ppm and limit of quantization 10,004 ppm.