THE ENHANCEMENT OF SECONDARY METABOLIT OF Orthosiphon stamineus Benth. IN ELICITATED CELL SUSPENSION CULTURE

• Main Author: Gusnelti, Yesi
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/37365
• Institution: Institut Teknologi Bandung

Orthosiphon stamineus Benth. is one of the potential medicinal plants that has been widely used to treat various diseases. O. stamineus are rich in of active secondary metabolites compounds such as sinensetin which is a marker compound of this plant. Alternative production of desired bioactive compounds from plants through biotechnological approaches, especially plant tissue culture has the great potential. This study aimeds to determine the increase in the content of secondary metabolites from suspension cultures of O. stamineus with elicitation using various types of elicitors. The research was initiated by the selection of the best plants then optimization of the sterilization method and plant growth regulators. The next stage was initiation of callus cultures from leaves as the explant. Callus culture was further used to initiate cell suspension cultures. Elicitation of cell suspension cultures were used to evaluate and analyze the content of secondary metabolites. Elicitors used were yeast extract (0.5; 1.0; and 2.0 mg / mL), chitosan (50, 100; and 150 mg / L), and methyl jasmonate (50; 100; and 200 ?M). In addition, isolation of major compounds of cell suspension cultures was carried out. The results showed that the best explant sterilization method was using Bayclin® 35% for 15 min and the best growth regulator was 2,4-D 0.5 mg / L. The use of elicitor to cell suspension culture does not affect the cell growth. There was an increase in the content of secondary metabolites after excitation in cell suspension cultures. Elicitors that can increase the content of sinensetin compounds at the highest level in cell suspension culture was chitosan (150 mg / L) up to 173%. Elicitors that can increase the level of compound X in cell suspension culture at the highest level was methyl jasmonate (100 ?M) up to 53%. In addition, new dominant compound appeared in cell suspension cultures (Compound X). This compound was still undercharacterization.