RHAMNOLIPID OPTIMIZATION THROUGH RECOMBINANT ESCHERICHIA COLI USING SYNTHETIC BIOLOGY APPROACH

• Main Author: Imaniar, Nina
• Format: Theses
• Language: Indonesia
• Subjects: Ilmu hayati ; Biologi
• Online Access: https://digilib.itb.ac.id/gdl/view/37391
• Institution: Institut Teknologi Bandung

The low stock of crude oil in Indonesia pushes the scientists to discover a new innovation in recovery technique, such as by usinga new biology agent with the ability to increase the volume of crude oil extraction in the reservoir. One of the propose solution is by using rhamnolipid as the surfactant molecule to reduce surface tension to facilitate extraction of remaining crude oil and optimize the recovery. The objective of this study is to characterize recombinant rhamnolipid obtained by synthetic biology approach and establish a biosensor system in rhamnolipid production. Rhamnolipid coding gene (RhlAB) is inserted into pUC57 under the control of the T7 promoter. Furthermore, this experiment used reporter gene mRFP as a biosensor to indicate rhamnolipid production. The quantitative analysis showed the highest concentration was obtained after 15 hours of incubation, pH 7 and temperature 37°C about 183,50 ?g/ml, 234,142 ?g/ ml and 202,351 ?g/ ml.. Furthermore, the qualitative analysis shows recombinant rhamnolipid is able to reduce the surface tension up to 23,67 Dyne/cm and has the drop collapse activity as the screening test of biosurfactant. The conclusion from the observation of rhamnolipid production based on detection of biosensor system showed that expression time between rhamnolipid production and reporter gene expression is unsynchronized, whereas the red color producing by reporter gene was produced at 21 hours after incubation when the rhamnolipid concentration starts to run out.