ISOLATION OF SIMPLE AROMATIC COMPOUNDS FROM STEM BARK OF CRYPTOCARYA DENSIFLORA THAT CAN BE USED AS SUPPORTING MEDIA TO IMPROVE OF ORGANIC CHEMISTRY LEARNING IN SENIOR HIGH SCHOOL

• Main Author: Sutrianah, Anna
• Format: Theses
• Language: Indonesia
• Subjects: Kimia
• Online Access: https://digilib.itb.ac.id/gdl/view/37721
• Institution: Institut Teknologi Bandung

Indonesia country is rich in natural resourches vary widely, especially plants that are commonly used as food, clothing and building materials. More than 80% of the world population use herbal medicine derived from plants. The popularity and development of traditional medicines are increasing in line with the tendency of people to return to nature. Therefore, a lot of researchers are interested in studying the Indonesian plants. One of Indonesian plant having potential as source of medicines is Cryptocarya. This genus of plant known locally as "medang-medangan" is one of general having high rank in evolution in Lauraceae family and it spreads over tropical and subtropical regions, including Asia, Australia, Melanesia, Southern Africa, and Brazil. Cryptocarya has economic values since its bark can be used for building materials and pulp. This plant is also used as traditional medicines, such as skin diseases, muscle-pain, joint-pain, headache, nausea, infection by fungi and bacteria. About 26 Cryptocarya species growing in Indonesia have been examined for their secondary metabolites. The main secondary metabolites are pyrones and flavonoids along with alkaloids, stilbenes, lignans, terpenoids, steroids and carboxylic acids. The secondary metabolites also have various bioactivities, such as anti-cancer, anti-malarial, anti-inflammatory and antimicrobial. One of Cryptocarya species growing in Indonesia is C. densiflora that its bark is used by people in Borneo as a medicine for poison from animals eliminating spasms, tuberculosis, dysentery and venereal diseases. The phytochemistry of C. densiflora has been reported previously. The stembark of C. densiflora growing in Malaysia has been reported to contain laurotetanine, reticuline and isocaryachin. While two akaloids, namely N-methyllaurotetanine and crychine have been obtained from the leaves. Furthermore, stembark of C. densiflora growing in Kalimantan has yielded reticuline and two terpenoid derivatives, namely linderane and pseudolinderadine. In this research, the isolation of secondary metabolites from stem bark of C. densiflora collected from Juanda Botanical Forest at Dago, Bandung. The isolation of secondary metabolites was carried out through a series of separation methods including maceration using acetone as solvent, fractionations and purifications by using vacuum liquid chromatography and radial chromatography techniques. In this research, two simple aromatic compounds idenfied as benzylbenzoate (130) and 4-allyl-2,6-dimethoxyphenol (131) have been successfuly separated from the acetone extract. The structures of isolated compounds were determined based on spectroscopic data including 1D NMR (1H, 13C) and 2D NMR (HSQC, HMBC, 1H-1H COSY). Both compounds were firstly reported from the stem bark of C. densiflora. The isolation techniques of natural compounds that have been conducted can be used as supporting media to enhance the organic chemistry learning in senior high school, therefore the chemistry, especially organic chemistry courses become more interesting.