Identification of Marine Bacterium Producing Chitinase
Chitinase is an enzyme hydrolyzing chitin polymer into chitin oligosaccharides and N N- acetylglucoseamine monomer. Chitinase can be used for many applications such as, emulsifier, fungicides, as an additive in livestock food and paper industries. The purpose of this research is to ide...
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| Format: | Final Project |
| Language: | Indonesia |
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| Online Access: | https://digilib.itb.ac.id/gdl/view/37862 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Chitinase is an enzyme hydrolyzing chitin polymer into chitin oligosaccharides and N N- acetylglucoseamine monomer. Chitinase can be used for many applications such as, emulsifier, fungicides, as an additive in livestock food and paper industries. The purpose of this research is to identify marine bacterium producing chitinase. The identification method was based on 16S rDNA sequence, using forward primer (Bactf1): 5’- AGAGTTTGATC(A/C) TGGCTCAG-3' and reverse primer (UniB1): 5’- GGTTAC(G/C)TTGTTACGACTT-3’. The result showed that 16S rDNA gene consisted of
1430 bp. In silico analysis by using the BLAST program and phylogeny tree showed that the bacterium had 99% nucleotide sequence similarity to that of Vibrio harveyi strain WG1702 (Gen Bank No. FJ768459.1). Meanwhile, the conventional microbiological identification based on morphology-biochemistry resulted in the bacterium belonging to Vibrio nereis. Based on both results above, the marine bacterium was identified as Vibrio sp.
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