Molecularly Imprinted Poly(Rhodamine B) Modified Carbon Paste Electrode for Voltammetric Determination of Uric Acid
Uric acid (2,6,8-trioxypurine) is a final product of metabolic breakdown of purine in human body found in body fluids, such as blood and urine. Higher uric acid concentration than normal level leads to conditions like gout arthritis and kidney stone formation, and indicates hyperuricemia and Lesch-N...
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| Format: | Final Project |
| Language: | Indonesia |
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| Online Access: | https://digilib.itb.ac.id/gdl/view/38010 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Uric acid (2,6,8-trioxypurine) is a final product of metabolic breakdown of purine in human body found in body fluids, such as blood and urine. Higher uric acid concentration than normal level leads to conditions like gout arthritis and kidney stone formation, and indicates hyperuricemia and Lesch-Nyhan syndrome. Analysis of uric acid can be performed using some methods, such as colorimetry, enzymatic, and voltammetry. In this research, a differential pulse voltammetry technique for uric acid determination has been developed using carbon paste electrode modified with Molecularly Imprinted Poly(Rhodamine B) as working electrode. Carbon paste electrode was modified using cyclic voltammetry by applying potential between -0,3 V – 1,0 V at a scan rate of 100 mV/s using 0,01 mM uric acid and 0,03 mM rhodamine B monomer solution in 0,1 M phosphate buffer solution pH 5 as supporting electrolyte. Calibration curve of uric acid showed good linearity at 0,01 mM –
1 mM with a detection limit of 6,36 µM. Determination of uric acid in human blood plasma and human urine samples displayed good results with percent recovery of 92,4% - 105,6%.
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