Production of Biosurfactant by Halophilic Bacteria Halomonas elongata BK-SM1 Using Olive Oil as Carbon Source for the Application as Antibacterials Agent

• Main Author: Hanifah Suprapto, Almira
• Format: Final Project
• Language: Indonesia
• Subjects: Kimia
• Online Access: https://digilib.itb.ac.id/gdl/view/38013
• Institution: Institut Teknologi Bandung

Biosurfactants are surface active agents produced by microorganisms as secondary metabolites under conditions of limited nutrient but rich source of carbon in the form of lipids. Currently, biosurfactants are widely used as an alternative to synthetic surfactants that generally are not environmentally friendly. One interesting application of biosurfactant is as the antibacterial agent. In the present study, the potential of Halomonas elongata BK SM1 as biosurfactant producer was explored. Biosurfactant was produced in this study in a media containing olive oil and urea as a carbon and nitrogen sources, respectively. The physicochemical characterization showed that the biosurfactants has the highest activity at pH 12 with emulsification index of 70.71%, and stable over a temperature range of 70-80 oC, as well as in a salinity range of 15-20%. Biosurfactants have the critical micelle concentration (CMC) of about 333 mg/l. Structural elucidation to the biosurfactant sample by FTIR, NMR and LCMS revealed that Halomonas elongata BK-SM1 produced two acidic biosurfactants, namely 5-hydroxy-9E-dodecenoate and 16-ethoxy-16-oxohexadecanoate. Biosurfactant potential as an antibacterial agent was evaluted by observing the growth of Escherichia coli for 24 hours at 37 oC and 150 rpm of areation in a growing medium containing various concentrations of biosurfactant. The result showed that microbial inhibition concentration (MIC) of the biosurfactant for the bacterial growth was observed at 0.5 mg/mL. Based on this result, biosurfactant produced by Halomonas elongata BK-SM1 exhibited high potential to be developed as an antibacterial agent.