Secondary Metabolites from Leaves of Cryptocarya pulchrinervia and Their Bioactivity against Murine Leukemia P-388 Cells

• Main Author: Nawar Ra'idah, Pramukti
• Format: Final Project
• Language: Indonesia
• Subjects: Kimia
• Online Access: https://digilib.itb.ac.id/gdl/view/38059
• Institution: Institut Teknologi Bandung

Cryptocarya is one of genus belongs to Lauraceae family that produces variety of secondary metabolites having biological activities that are beneficial to human. Several species of the Cryptocarya have hard woods that are used as building materials and pulp. In addition, this genus is used as traditional medicines as headeache, nausea, and pain medication. It Cryptocarya mainly distributes in tropical and subtropical regions and can be found in Asia, Australia, America, and Africa. This genus has high rank in evolution, therefore it assumed that this genus can produce many types of secondary metabolites having unique skeletons that makes this genus is interesting to be studied. One of Cryptocarya growing in Indonesia is Cryptocarya pulchrinervia. The phytochemistry of leaves of C. pulchrinervia has been previously reported that has succesfully isolated three compounds, identified as pyrones derivatives (CP-1 (29), CP-2 (1), CP-3 (30)) proposed as new compounds. Moreover, compounds of CP-1 and CP-2 have high cytotoxicities against murine leukemia P-388 cells (IC50 ~ 3.0 ?g/mL). However, the research has still not accomplished yet, therefore the continuation of research has to be conducted to obtain more compounds. Then, the isolation of secondary metabolites have been carried out by several of separation methods, including extraction, chlorophyll separation, and purification by various chromatographic techniques i.e. vacuum liquid chromatography, radial chromatography, and gravity columns chromatography. Furthermore, the structure of the isolated compound were identified based on spectroscopic data including NMR (1H, 13C, HSQC, and HMBC). In this research, six compounds have been obtained, two of them were characterized as pyrone derivatives, i.e. CP-2 (1) and a mixture contain CP-2 (2:1). While, two other compounds were suggested as pinocembrin (5,7-dihydroxy flavanones), i.e. a flavanon derivative) (2) and N-trans- feruloyltyramine (an amide) (3) which have been reported from other Cryptocarya species. The bioactivity of the pyrones derivatives against murine leukemia P-388 cells showed that CP-2 (1) and a mixture contained CP-2 were active, with the IC50 value of 3.03 ?g/mL and 3.82 ?g/mL, respectively. In addition, the pinocembrin (2) and N-trans-feruloyltyramine (3) have been reported previously to be not active (IC50 59.3 µg/mL and 37.4 µg/mL, respectively). CP-2 (1) is a major compound and is suggested to be the leading compounds for anticancer.