CARBON PASTE ELECTRODE MODIFIED MOLECULARLY IMPRINTED POLY(METHYL RED) FOR VOLTAMMETRIC DETERMINATION OF PARANITROPHENOL
Paranitrophenol (PNP) is widely used in chemical industries to make paint, pesticide and drugs. PNP waste from industry is toxic and can be harmful to living things when it is exposed to environment. PNP concentration in environtment are normally very small, so that it requires sensitive analytica...
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| Format: | Theses |
| Language: | Indonesia |
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| Online Access: | https://digilib.itb.ac.id/gdl/view/38253 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Paranitrophenol (PNP) is widely used in chemical industries to make paint, pesticide and drugs. PNP waste from industry is toxic and can be harmful to living things when it is exposed to environment. PNP concentration in environtment are normally very small, so that it requires sensitive analytical method for PNP determination. Several analytical methods had been developed for PNP such as UV-Vis spectrophotometry, High Performance Liquid Chromathography (HPLC), and voltammetry methods. Voltammetry has several advantages compare to other methods. It is simple, sensitive and selective. In this study, we developed molecularly imprinted polymer (MIP) modified carbon paste electrode (CPE) for PNP determination. MIP was plated on CPE using cyclic voltammetry (CV) technique with 5 potential cycles at potential range of -1,5 V – 1,5 V and scan rate of 100 mV/s from solution containing PNP, methyl red and phosphate buffer of pH 7. The concentration of PNP and methyl red was 1 mM and 5 mM, respectively. MIP was formed by removing trapped PNP using 30 potential cycles at potential range of -1,5 V – 1,5 V with scan rate of 100 mV/s in acetate buffer of pH 4,5. Square wave voltammetry (SWV) technique was used for PNP determination in acetate buffer of pH 4,5 as supporting electrolyte. Repeated measurement using the modified electrode gave %RSD of 6,14% (n=30). Linear calibration curve was found in the concentration range of 5 µM – 40 µM with detection limit of 4,66 µM. PNP determination of spiked river samples resulted in percent recovery of 86% – 105% showing a good accuracy of the method.
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