PREPARATION OF CANDIDATE OF REFERENCE MATERIAL FOR DETERMINATION TOTAL SELENIUM IN POTATOES BY HYDRIDE GENERATION-QUARTZ FURNACE ATOMIC ABSORPTION SPECTROPHOTOMETRY (HG-QFAAS)
Reference material is a material that has certain properties which is quite homogeneous and stable. Nowadays, reference materials with the matrix is necessary for the purpose of analysis of a sample including food samples but the availability is limited, expensive and difficult to obtain. Referenc...
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| Format: | Theses |
| Language: | Indonesia |
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| Online Access: | https://digilib.itb.ac.id/gdl/view/38268 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Reference material is a material that has certain properties which is quite homogeneous and stable. Nowadays, reference materials with the matrix is necessary for the purpose of analysis of a sample including food samples but the availability is limited, expensive and difficult to obtain. Reference materials have an important role in the data accuration. Selenium is a unique element, because it has two opposite properties, as an essential nutrient for living things but in larger doses it can be toxic. Potato tubers (Solanum tuberosum .L) as one of the staple food consumed by people in the world, becoming an object for this research. The appeal of potato located in the tuber which is rich in carbohydrates and high nutritional value. The aim of this study is for preparing candidate reference materials for the determination of total selenium in potato tubers using hydride generation quartz furnace atomic absorption spectrophotometry (HG- QFAAS). Samples were taken from the potato plantations located on the Dieng plateau, Wonosobo, Central Java. Analysis was conducted on the moisture content, ash content, and the total content of Se. Methods for determination of moisture content, ash, and the total content of Se validated. Validation methods include the detection limit, linearity, homogeneity, stability, precision and accuracy. Analysis of moisture and ash content carried out by gravimetric method. Moisture content and ash content on average of 10 samples measured value in a row (8.24 ± 0.03)% and (4.67± 0.39)%. The analysis of total content of Se was determined using hydride generation - quartz furnace atomic absorption spectrophotometry from destruction of the sample with a strong acid. Homogenity testing was analyzed by one way ANOVA, showed that the raw and ripe samples were homogenous and no significant differences between repeatation. Precision of 1.20% on the first day, 5.42% on the second day and 2.10% on the third day. The linear area measurements for the calibration plots showed good linear relationship in the concentration range of 2-20 µg/L with the correlation coefficient (R2) of 0.9986. The limit quantitation of 1.66 µg/L. Measurement of total Se concentration in the sample giving the percentage recovery of 98.30 %, showing good accuracy.
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