PRODUCTION AND APPLICATION OF LEVAN AS IN BASE MATERIAL OF METAL ION NANOPARTICLES FOR ANTIOXIDANT AND ANTIBACTERIAL

• Main Author: Mahbuba Saleha Amari, Muzayana
• Format: Theses
• Language: Indonesia
• Subjects: Kimia
• Online Access: https://digilib.itb.ac.id/gdl/view/38552
• Institution: Institut Teknologi Bandung

l,e mn is exopol{fructose which is producedfrom several types ofplants.fungi. and bacteria. Le mn has been widely applied in industrial.fields. especially in health indusrry used as a base for synThesis ( (metal ion nanoparticles. Levan symhesis invoh•es an enzymatic reaction hy lemnsucrase (E.C. 2.-1. 1. 10). In thi s study. the production of recombinant levansucrase Lsbl-bk1 was carried out in E coli BL21(DE3)plysS which had carried the leFansucrase encoding gene (lsbl-bkl). Based on the e /ectrophoregram results ofSDS-PAGE. the size of Lsbl-bk 1 obtained rangedfrom 50 kDa. The spec(fic activity of recombinanT levansucrase Lsbl-bk1 produced /.-15 units mg and was able to produce levan in vitro of -1.28 mg mLfrom a production medium of 100 mL containing 12% (w 1) sucrose. TheIR spectmrn of lemn synthesized by Lsbl-bk1 ha s a profile similar to the standard IR lemn spectmmfrom Ent•inia herbicola. Lemn produced in Pitro is used as a stabilizing agent and reducing agenT in the mam facwre of metal ion nanoparticles. In thi s study. jil•e types f leran-based metal ion nanoparticles (lemn-xNPs) were synthesrzed. namely leran-Fe: NPs. lemn-Ag NP. levan-Cu NPs. levan-Co: NPs. and lemn-Zn NPs. Each lemn-xN Ps were characteri::ed using UV-Vis. FTIR. EDS. andTEM. The result s of analysis using a l TV-Vis spectrophotometer showed that the maximum wm•elength of complemenlat)l colors of levan-Fe2 +NPs solution. levan­ Ag NP. lemn-Cu NPs. levan-Co2 NPs. and levan-Zn NPs respecth•ely were 301 nm. -1-12 nm. 298 nm. 303 nm. and 288 nm. 7he Ff'JR resultsfrom each le mn-xNPs also showed IR spectrum prfiles that were in accordance with the le mnfunctional group after analysis through a combination f only lemn IR spectmm. metal 1R spectrum. and lR le van-xN Ps spectnmr . This means that lemn is found on the sm:face of the nanoparticles (as stabilizers). The composition of metal elements in le mn-based metal nanopanrc/es ha s also been analyzed using EDS. 1he EDS mapping result s on lemn-xNPs show that there are 20% Cu. 1-1% fe. -16% Ag metal. /3% metal Co. and 35% Zn metal detected on the sm:face of rnetal nanoparticles. Tl:M results show the spherical morphology metal ion nanoparticles with sizes ranging from less than 100 nm for all /evan-xNPs. The confirmed le mn-based metal ion nanoparticles. tested their potential as an antioxidants and as an antibacterial. IC5n mlue is the minimum concentration mlue of an antioxidant solution to reduce radical compounds by 50%. This mlue is a reference for finding le mn-based metal ion nanoparticles thu1 hm•e the potenrial to be antioxidams. Based on 1he JC5o mlue obtained in this study. the le mn-based metal ion nanoparticles which hm•e the potential to be the active antioxidant is lemn-Fe 2 NP s with an JC5o value 0.5 mg mL. H owe1•er. /e mn-Cu NP s has a high percemage inh1bilion of DPPH radical compoundaround 95% Thi f; mlue is higher than the percent inhibition of radical compounds by a scorbic acid (85%). Whereas the potential to he an antibacterial are le mn-Co•' NPs and leran-Ag NPs. J'he Jdent[/icatl on of annbactenal determination was based on clear ::one diameter data from Escherichia coli BL2 I (Gram negatil•e bacteria). Pseudomona s aemginosa (Gram negatiJ•e bacteria). and Sta phylococcus ariel/ a (Gram positil•e bacteria) test bacteria. Further identlfication 1ms carried out on leran-C'o2 ' NPs and leran-Ag N P s to see the most sensiti1•e antibacterial actirity against Gram positive and Gram negati1•e test hacte1ia. 'The resulls showed that le mn-Co2 NPs had the mosl sensifh•e antibac1erial ac1ivi1y 10 E. coli BL21 (G-) u•i1h a rninimum inhibiwry concencrwion of 5 pg mr which ga Pe a clear zone diameter of 25 mm. While lemn-Ag NPs has the m ost sensitive antiba cte rial ac ti vit y t o S. arl etla (G ) with a minimum inhibitory concentraTion of I pg mL which gil•es a clear zone diameter of I-I mm. The rew / H hmred that/emn-haed metal ion nanoparticles (Fe 2+ . Ag+. Zn 2+. Cu+. and CoJ ) were successfully synthesi::ed and ha1•e been tested rhar le mn-Fe2 NPs and /evan-Cu NP s have potential as ne w anlioxidant materials and leran-Co2 NPs with lemn-Ag NPs ha1•e the potential as a new antibacterial material.