Immobilization of a-Amylase Enzyme on Cellulose Acetate (CA) Matrix

• Main Author: Nur A Rahman, Surya
• Format: Final Project
• Language: Indonesia
• Subjects: Kimia
• Online Access: https://digilib.itb.ac.id/gdl/view/38572
• Institution: Institut Teknologi Bandung

Cellulose acetate can be used as a matrix for enzyme immobilization. The surface activation of cellulose acetate aims to convert the less active ester group into a more active carboxylic group so that a CO\,alent bond bet-ween cellulose acetate and an enzyme can be formed. In this study. activation -was carried out using HS04, Nal04, and H 2S04-Nai04. The FTIR spectrum did not show a significant change in the surface of cellulose acetate after activation. However, after testing the enzyme activity gave positive results v. ith the best results using 3 M H 2S04. Variations in enzyme contact time v.ith cellu l ose acetate, starch contact time -with cellulose acetate-enzymes. and starch concentration have been carried out. The variation of enz) me contact time was carried out in the period of 0.5-6 hours in borate buffer 0.1 M pH 9 and optimum contact time of 3 hours -was obtained . Variation in contact time for starch was carried out at a time range of 5-120 minutes in acetate buffer 0.1 M pH 5 with I hour enzyme contact time and optimum contact time of 60 minutes. Variations in starch concentration were carried out at a concentration range of 0.025-1% (w/v) and the results showed that the increasing concentration of starch increased the concentration of reducing sugars. The optimum conditions for starch concentration cannot be determined because it is constrained by making higher concentrations of starch solutions. -which are increasingly difficult to dissolve. At optimum conditions. enzyme contact time of 3 hours. starch contact time of 60 minutes. and starch concentration of 0.2% (w/v) obtained reducing sugar concentration values of 1588.76±24.33 ppm per 0.2 gram of activated cellulose acetate. Repeated use with optimum conditions was carried out three times and obtained the second. third, and fourth reducing sugar concentration values, respectively 538.37±1.46; 620.55±8.95: 536.41±8.56 ppm per 0.2 gram of activated cellulose acetate. Morphological characterization was also carried out on the surface of cellulose acetate before and after enzyme immobilization v.-ith 12.000 times magnification.