ISOL4 T/OjY OF SECO:VDARY 1UETABOLJTES, CHE.ll/CAL TRANSFORMATIO.V OF a-PYRONE ASD CI'TOTOXJCJT'f TEST 0.\ CRYPTOCA RYA PULCHRL'VERV IA (LACRACEAE)

• Main Author: Nawar Ra'idah, Pramukti
• Format: Theses
• Language: Indonesia
• Subjects: Kimia
• Online Access: https://digilib.itb.ac.id/gdl/view/38802
• Institution: Institut Teknologi Bandung

( ryptocar_w zs one of the genus belongs to Lauraceae fcmzil_v which produces various secondary metabolites with broad bioactzvities. Sorne Cryptocar_va species Jun•e a/.<.;o been used as tradztionul remed1es as pain relief, 10 treat nausea, and to cure inft!ctions caused by fimgi. These bioacttvtttes are related with secondary metabolites contained in Cr;ptocarya. The mam secondmy metabolites from thi!> genus ure flavonoid:;, pyrones. and alkalouls. Among the three secondary metabolite rzroups, a-pyrone.• from CtJplocw:m having dihydro sl_HJ'l skeleton have been reported posses.sing various hwacth•ities 1.e. anticancer, cmliil?f/ammation, antifzmgal, unt imalarza, and antivirus. nus group has also heen reported from several lndoneswn Cryptocarya. One of indonesian Cryptocurya is C. pulc/1rinervia. Previous investigunons reponed that leaves of C. pulchrinervw yielded two pyrone derivatives, i.e. a new compound CP-1 and cryplobrachylone (•. one flavanone (pinocembrin), dan one amide derivative {N-trans­ feruloyltyrumine). CP-1 and cryptobrachytones C were active against murine leukemw P-388 cells• (!C5o 3.03 und 2.67 fl.g mL), while pinocembrin and N-truns­ .ferulo_vltyramine were inucth•e (1('50 -+,0 pg m!J Cryptobrachytones C wu.s uctuul \' recentZv reported as new compound from C. bracl vtl vrsa and the main compound rn C. pulchrinervia (200 mg I kg dt)' leaves). Therefore, in this research tlze continuining investigation ofsecondat:v metabolites to obtain other compozmd•, ha\ been carried out as well as chem1ca/ tranformution of Ct )ptobrac/ •vtones C tu increase the hiouctinty uguinst murine leukemw P-388 cefls.Fzmhermore, seven cump01md\ have been obtained consisting four p_1-rones derimtn•es includmg threl! nt!u• wmpound\• t.e. fS)-rugulactone. CP--1. and CP-l. and a known compound •;. ryptohruch_vtones (•. alung H"ith three known am{(/es Jenvatl •es, r.e. N-tram- 1 rult\-llr:. plumin.:, .\'-;runs-firulo)•1-3-metoxy-ll ramine and X -tram- jruloyltyrumme. 1\Jorl!on:r. three ch::nucul tram'frwmatmm (reduct1m1, ac..:t_1•fat1 m, oxa -_\liclw.-:l t..yc.h::ution) _;,, o:1ptubruchytunus C hemulso been conduc1ed. The rvduct/017 reactwn on cl:llJlobrachytmu:'' C ({[fimled RR-1 and RR- acef).lat wn produced R-4 - I and R-4 -]. Oxa-,\!iclwel crclr:utwn on u:\ptohracllJ!ones ( J zeldeL/ !G..')- f. 1he cytotOXICIIJ examination on 1\oluted vmpotmd.' ami ch micul tnm'>}orrnutwn compound,• showed thut (\";-ruguluctoJ'L iW\ ,Jt:ln{! ((JC511 U-10 J19 mL; ll'iule C'P-...J 1ws 111th."tn•e. in wldltlon, RR-:l R -1. and R-l-llt er<? actl'.'e 11 uh /( .'t l IJ( 1.3(}, 3.50 dun :!.30 ,ug ml, ,e,pe.;tm! .lm u.! Ill derivwil•es. RR- I and R5:- I were im.Jcflve (!C5u -i.0 f1B mL). The chem1ca!. lrun fhrmuted compound !U?-2, showed mcreal'ing actin(v compared ro cryptobruchytones C. In conclusiOn, compound RR-2 and (.))-rugulactone were lh<! two compoundlwving the highest potentwlto be ant1cancer agl.!nt.