Optimation of Essential Oil Yield from Patchouli (Pogostemon cablin var. Patchoulina 2) Stem and Shoots Through Lignocellulose Biodegradation of Distillation Feeds

• Main Author: Shalsabila, Syakina
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/39235
• Institution: Institut Teknologi Bandung

Patchoulina 2 is a variety of Pogostemon cablin which results from somaclonal modification of patchouli Sidikalang variety with higher productivity, which is 12,56 ton/ha/year. This research aims to compare the productivity of Sidikalang and Patchoulina 2, and to study the effect of Patchoulina 2 pre-treatment on the yield of patchouli oil and patchouli alcohol content. Pre-treatment is carried out in the form of lignocellulose degradation of distillated feed by P. chrysosporium and T. viride. Patchoulina 2 has higher biomass productivity and patchouli oil yield compared to Sidikalang. Sidikalang stem-shoots can produce patchouli oil with the yield of 1.22% and patchouli alcohol content of 54.58%, while the yield of Patchoulina 2 stem-shoots patchouli oil is 1.41% with patchouli alcohol content of 74.52%. Lignocellulose degradation of patchouli stem-shoots results in the increase of patchouli oil production. T. viride can degrade hemicellulose and cellulose of patchouli stem-shoots to 23.88% and 14.88%, which is resulting in the increase of patchouli oil yield to 1.77% with patchouli alcohol content of 72.77%. Meanwhile, P. chrysosporium can degrade hemicellulose and cellulose to 20.43% and 15.69%, which is resulting in the increase of patchouli oil yield to 1.89% with patchouli alcohol content of 71.16%. Pre-treatment of Patchoulina 2 stem-shoots by P. chrysosporium results in the highest patchouli oil productivity among the other variations conducted in this study.