UJI AKTIVITAS ANTIHIPERGLIKEMIA DAN ANTIOKSIDAN NANOEMULSI EKSTRAK KULIT KAYU RARU [MESUA HEXAPETALA (HOOK.F.) P.S.ASHTON]

• Main Author: Fernandez Nababan, Tommi
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/39782
• Institution: Institut Teknologi Bandung

Raru [Mesua hexapetala (Hook.f.) P.S. Ashton] is a term for the type of bark added to palm sap which aims to improve taste, alcohol content and preserve traditional tuak drinks. The literature study shows that raru n-hexane extract has in vitro antihyperglycemic activity. The aim of this study was to find a stable and homogeneous nanoemulsion formula with a globule size below 200 nm, and to compare the antihyperglycemic and antioxidant activity of raru n-hexane extract formulated in nanoemulsion with ordinary emulsions and extracts. Nanoemulsion is done by a combination of high shear homogenization and ultrasonication methods. From the research, the formula that produces nanoemulsion which is in accordance with the target is Formula 2, consisting of a combination of Cremophor RH 40 (10%), propylene glycol (2.5%), polyethylene glycol 400 (5%), glycerin (2.5%) and oleic acid (4.75%). Size of nanoemulsion globule 101.5 nm, PI 0.46, stable in temperature stability test 4?C, 25?C, and 45?C, freeze and thaw test, and centrifugation test. The results of testing the antioxidant activity of vitamin C, raru extract, and raru nanoemulsion were 7.69 ppm, 129.39 ppm and 129.34 ppm, respectively. Antihyperglycaemia nanoemulsion and emulsion activity test with glucose tolerance method showed that nanoemulsi preparations were able to reduce blood glucose levels in test animals significantly compared to the control group at T90 and T120 (p <0.05) and T90,120, and 150 (p <0.075 ), while emulsion preparations were able to reduce blood glucose levels in test animals but were not significant compared to the control group. Statistical tests on the performance of nanoemulsion with ordinary emulsion showed significant differences with p <0.075 at T120 and T150 points.