Hydrolyzed Protein Production from Defatted Biomass of Black Soldier Fly (Hermetia illucens) Cultivated in Avocado and Avocado – Soybean Curd Residue Substrate

• Main Author: Muzakki Sabila Hidayat, Ghassani
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/39835
• Institution: Institut Teknologi Bandung

Hydrolyzed protein, a result of partial hydrolysis, has been known to contain more nutritional value and can be used more efficiently than the unhydrolyzed amino acid. Protein from insects has the potential to be utilized as an alternative that is more economic and environmental. The goal of this research was to determine the yield, productivity and characteristics of hydrolyzed protein from Black Soldier Fly biomass that was cultivated using rotten avocado and rotten avocado-soybean curd mixture (1:1). The cultivation was carried out for 27 days at temperature of 19- 38,5oC and humidity of 34,9-99,2% with a light intensity of 0,03-46,8 W/m2. Defatted biomass was obtained from the extraction of Black Soldier Fly using an immersion method with n-hexane and then hydrolyzed in water, bromelain enzyme (5%), and incubated for 13,5 hours at pH 8 in a waterbath shaker stirred at 150 rpm. The hydrolyzed protein was then centrifuged at 4000 rpm. The protein fraction from Black Soldier Fly biomass increased 1,4 times as the result of the immersion and centrifugation. The yield and productivity of hydrolyzed protein from avocado and avocado-soybean curd treatment were 17,88% ; 0,036 g/ml/batch and 12,07% ; 0,024 g/ml/batch, respectively. Amino acid composition in the hydrolyzed protein from avocado and avocado-soybean curd treatment were dominated by essential amino acids such as lysin (9,14 ; 9,4%), valine (7,93 ; 8,5%) and leucine (6,14 ; 7,2%) that are vital to regulate metabolism for mammals, whereas the non-essential amino acids were dominated by glutamic acid (18,2 ; 16,5%) and alanine (16,74 ;18,1%).