A Study on Lipopeptide Biosurfactant as Potential Antibiofilm Agent in Nuclear Reactor Cooling Water System
Water is the preferred medium for heat removal fluids in many industries as well as nuclear power plants. The water used in the cooling system is generally overgrown by biofilm forming bacteria. The growth of biofilms on the surface of the heat exchanger in the cooling water system can result in red...
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| Format: | Final Project |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/39889 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Water is the preferred medium for heat removal fluids in many industries as well as nuclear power plants. The water used in the cooling system is generally overgrown by biofilm forming bacteria. The growth of biofilms on the surface of the heat exchanger in the cooling water system can result in reduced heat exchange efficiency. Biofilm growth also allows biofouling and biocorrosion to occur which can cause losses in the operational process. Biocides have been the main weapon used to control biofilms in cooling water system. However, their use is gradually restricted due to their toxic effects on the environment. This study aims to determine the potential of lipopeptide biosurfactant produced by Bacillus sp. to be used as an anti-biofilm agent that is more environmentally friendly. In addition, minimum inhibitory concentration (MIC), minimum biofilm inhibitory concentration (MBIC), and minimum biofilm eradication concentration (MBEC) were determined to obtain effective concentrations in dealing with biofilms. The biosurfactant potential as an anti-biofilm agent was observed based on the inhibitory ability of planktonic cell growth, anti-adhesion, and biofilm eradication. Isolation and screening of biofilm forming bacteria from cooling water used in nuclear power plants were performed. Biofilm-forming isolates were tested with biosurfactants at various concentrations and compared with commercial biocides. Growth inhibition and MIC values were determined by the total plate count (TPC) method. While the activity of anti-adhesion and biofilms eradication along with their MBIC and MBEC were determined based on the wet weight of the biofilms. These wet weight results were also confirmed visually by using confocal laser scanning microscopy (CLSM). The results of TPC calculations showed biosurfactant was capable of inhibiting planktonic cell growth by up to 60.43% while commercial biocide was able to inhibit growth up to 99.91%. Biosurfactant also has anti-adhesion activity of 72.97% while there is no anti-adhesion activivty observed in commercial biocide. Eradication of biofilms with biosurfactant reached 76.92% while commercial biocides were only able to eradicate up to 69.23%. From the results of the analysis, the MIC, MBIC, and MBEC values were 62.5 ?g/mL, 62.5 ?g/mL, and 125 ?g/mL respectively. This study showed that lipopeptide biosurfactant produced by Bacillus sp. has the promising potential to be used as an anti-biofilm agent for biofilms formed by isolates of nuclear reactor cooling water systems.
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