EFEK URIKOSTATIK EKSTRAK AIR KALIKS ROSELA (HIBISCUS SABDARIFFA L.), EKSTRAK AIR DAUN TEMPUYUNG (SONCHUS ARVENSIS L.), DAN KOMBINASI KEDUANYA PADA TIKUS WISTAR JANTAN

• Main Author: Khotimah, Khusnul
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/39898
• Institution: Institut Teknologi Bandung

Uric acid is a product of purine metabolism in the body. Uric acid levels that exceed normal (hyperuricemia) can form urate crystals which may cause several diseases, including gout arthritis and kidney stone formation. In some studies, water extract of calyces roselle (Hibiscus sabdariffa L.) has a uricosuric effect and the water extract of field sowthistle leaves (Sonchus arvensis L.) has a uricostatic effect. This study aims to determine the uricostatic effect of the combination of both extracts. Uricostatic effect was studied in vivo using male Wistar rats which were divided into six groups, positive control group, roselle 40 mg/kgBW, field sowthistle 40 mg/kgBW, combination group 40-40 mg/kgBW, combination group 20-20 mg/kgBW, and allopurinol 9 mg/kgBW as the reference. The test was carried out through two stages which was the induction stage and the administration of the test substance. The induction stage was carried out by giving high purine food for 7 days. The administration of the test substance was carried out for 4 days by giving the test substance according to the test group. All the test groups had a uricostatic effect which was indicated by the p value of uric acid levels which was significantly different (p<0.05) compared to the positive control group. The resistance value of each rosela test group was 40 mg/kgBW, field sowthistle 40 mg/kgBW, a combination group 40-40 mg/kgBW, and combinations group of 20-20 mg/kgBW were 21.08%, 41.24%, 48.88%, and 40.18%. The uricostatic effect of the combination extract 40-40 mg/kgBW and the combination of 20-20 mg/kgBW was not significantly different from the single extract but were significantly different from allopurinol. Thus, the combination extract had an additive uricostatic effect on its single extract and was lower than allopurinol.