ISOLASI DAN UJI AKTIVITAS ANTIMIKROBA METABOLIT SEKUNDER ASPERGILLUS AMSTELODAMI ASAL SPONS LAUT

• Main Author: Sapitri, Nita
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/40023
• Institution: Institut Teknologi Bandung

Antibiotics are compounds that used for inhibit growth or kill a pathogenic microorganism in the human body. However, the ability of these antibiotics will be disturbed by the mechanism of resistance in microorganism that increase day by day. Various efforts were made to reduce the number of resistance, one of which was to find new antibiotics. The aim of this research was to isolate and determine antimicrobial activity of secondary metabolite of endophytic fungi from the sea sponge from Seribu Island, namely Aspergillus amstelodami. Aspergillus amstelodami was fermented by static method for 30 days to obtain these secondary metabolites. Media and mycelium produced from fermentation were extracted with ethyl acetate. Media and mycelium extract were monitored by analytical thin layer chromatography using ethyl acetate : hexana (1:9) as mobile phase. The antimicrobial activity of both extracts were determined by agar diffusion method. Media extract had the best antimicrobial activity with inhibition zone 8 mm against Escherichia coli, 10 mm against Staphylococcus aureus, and 7,8 mm against Candida albicans. The media extract was fractionated by classical column cromatography with increased solvent polarity. The 27th fraction had the best antimicrobial activity with inhibition zone 21.5 mm against Escherichia coli, 23.15 mm against Staphylococcus aureus, and 18 mm against Candida albicans. The 27th fraction was identified using LC-MS with Electrospray Ionization. The ESI-MS mass spectrum shows that the fraction is predicted contains patulin and ochratoxin A which have antimicrobial activity, and other compounds with molecular weight 226 g/mol and 285 g/mol. Thus, the fraction of secondary metabolites of Aspergillus amstelodami has the potential to be developed as an antimicrobial agent.