UJI TOKSISITAS SUBKRONIK KOMBINASI EKSTRAK AIR DAUN TEMPUYUNG (SONCHUS ARVENSIS L.) DAN KALIKS ROSELA (HIBISCUS SABDARIFFA L.) PADA TIKUS WISTAR

• Main Author: Shifanurani N, Syahnas
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/40207
• Institution: Institut Teknologi Bandung

Sowthistle (Sonchus arvensis L.) has been used traditionally to treat various diseases such as swollen, intestinal infection, dysentery, hemorrhoids, inflammation, lethargy, aches, kidney stones and rheumatism. Meanwhile, roselle (Hibiscus sabdariffa L.) has been used as antiinflammatory, antiseptic, antibacterial, astringent, analgesic, decresing cholesterol and uric acid level. In preclinical study, the combination of sowthistle and roselle were claimed to have antihiperuricemia. Sowthistle had LD50 value of >5000 mg/kg bw and roselle had LD50 value of > 5000 mg/kg bw. The value of LD50 of the combination of both sowthistle and roselle is >2500-2500 mg/kg bw. There’s no data supporting subchronic safety of the combination. The aims of this study is to determine the toxicity of the combination of water extract of leaves of sowthistle and roselle calyces and the delayed effect after 30 days of the given test being stopped. Subchronic toxicity testing method was carried out based on Perka BPOM RI No.7 of 2014. The test groups consist of (1) combination of sowthistle and roselle extract in 40-40 mg/kg bw, (2) combination of sowthistle and roselle extract in 200-200 mg/kg bw, (3) combination of sowthistle and roselle extract in 500-500 mg/kg bw, (4) control group and (5) two satellite groups. The results showed no significant difference of the useage of combination extracts in all dose groups compared to the control group. There were also no significant differences after stopping the useage of combination test extracts at high doses based on hematological parameters, blood biochemistry, urine analysis, habits, organ index, body weight gain, organ macropathology and histopathology. In conclusion, the combination of water extract of sowthistle leaves and roselle calyces is not toxic to use until 500-500 mg/kg bw test dose for 90 days and there were no delayed effect after 30 days of the given test was stopped.