AKTIVITAS ANTIOKSIDAN EKSTRAK DAUN, BATANG, DAN AKAR SELEDRI (APIUM GRAVEOLENS L.) DENGAN METODE DPPH DAN ABTS
Free radicals are atoms or molecules with one or more unpaired electrons in their outer orbitals, therefore, they need to take electrons from other molecules, which creates an imbalance of other molecules and initiates a chain reaction leading to health problems such as degenerative diseases, cel...
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| Format: | Final Project |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/40214 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Free radicals are atoms or molecules with one or more unpaired electrons in their outer orbitals,
therefore, they need to take electrons from other molecules, which creates an imbalance of
other molecules and initiates a chain reaction leading to health problems such as degenerative
diseases, cell damage, tissue damage, and cancer. Antioxidants can reduce the negative effects
of free radicals. Antioxidant can be found in various plants, such as celery (Apium graveolens
L.). The objectives of this research were to study antioxidant activity of leaves, stem, and root
extracts of celery using DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS [2,2'-azino-bis (3-
ethylbenzothiazoline-6-) sulfonic acid)]; determine total phenolic and flavonoid content;
analyze the correlation between total phenolic and flavonoid with IC50 DPPH and IC50 ABTS, and
also analyze the correlation between DPPH and ABTS in sample extracts. The leaves, stems, and
roots of celery were extracted by reflux method using increasing polarity solvent, e.g. n-hexane,
ethyl acetate, and ethanol. Determination of IC50 DPPH, IC50 ABTS, total phenolic and flavonoid
content were performed by uv-visible spectrophotometry. Correlation of total phenolic and
flavonoid content with their IC50 of DPPH and IC50 of ABTS and also correlation of two methods
were conducted by Pearson method. The highest total phenolic content in ethanol leaves
extract (40.25 g GAE/100 g), while the highest total flavonoid content was in ethanol stems
extract (29.56 g QE/100 g). Antioxidant activity of leaves, stems, and root extracts of celery
showed IC50 DPPH in the range of 1.63 ????32.24 ?ã??????????????íð?± ?í± IC50 ABTS was in the range of
4.12 ?????????????????????????ã????????????????•??í±???ð£ í• ?±ã•?ð?± •? ?ðã?ð»ð£•??£???±?•?ð???ð?í IC50 DPPH of
£±?±????????±•?±???????±??????•??????±???•£??•? ?ð?í ?????50 !??????????» £±?±?????????±??•? ?????±???•£??????
Furthermore, total flavonoid had negative and significant correlation with IC50 DPPH and IC50
!??????????» £±?±????????±•?±???????±??????•? ?????±???•£??????IC50 DPPH and IC50 ABTS values showed
???ð?ð?± £???±?•?ð??ð?£±?±?????????±??•? ?????±???•£??????!???•???± ±???•£???±?± ?±?y strong
antioxidant. In general, the phenolic and flavonoid compounds contributed together in
antioxidant activities of sample extracts. DPPH and ABTS methods gave linear result in
antioxidant activity of stems and roots extracts of celery.
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