ANTIOXIDANT ACTIVITIES OF LEAVES EXTRACTS OF FOUR KINDS SYZYGIUM USING DPPH AND CUPRAC METHODS
Background and purposes: Syzygium is one of the plants that widely used as a natural antioxidant by the community. Many kinds of Syzygium in Indonesia makes researcher interested to compare antioxidant activity of selected Syzygium plants. The purposes of this study were to determine the antioxid...
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| Format: | Theses |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/40224 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Background and purposes: Syzygium is one of the plants that widely used as a
natural antioxidant by the community. Many kinds of Syzygium in Indonesia makes
researcher interested to compare antioxidant activity of selected Syzygium plants.
The purposes of this study were to determine the antioxidant activity of leaves
extracts from four kinds of Syzygium using DPPH and CUPRAC methods,
determine total phenolic and flavonoid content, analyze the correlation between
total phenolic and flavonoid content with IC50 DPPH and EC50 CUPRAC, and
correlation between IC50 DPPH and EC50 CUPRAC. Methods: Extraction by reflux
with three different solvent polarity. Determination of IC50 DPPH and EC50
CUPRAC, total phenolic and total flavonoid content were carried out by uv-vis
spectrophotometer. Correlation of total phenolic and flavonoid content with IC50
DPPH and EC50 CUPRAC and the correlation between the two antioxidant
methods were analyzed using Pearson’s statistical method. Results: The highest
antioxidant activity by DPPH method was ethanol leaves extract of Syzygium
jambos with IC50 1.78 µg/mL and the CUPRAC method was ethanol leaves extract
of Syzygium malaccense with EC50 10.31 µg/mL. The highest total phenolic content
was shown by ethanol leaves extract of Syzygium jambos (64.36 g GAE/100 g) and
the highest total flavonoid content was shown by ethyl acetate leaves extract of
Syzygium malaccense (16.44 g QE/100 g). Total phenolic content of all samples
had negatively and significant correlation with their IC50 DPPH dan EC50
CUPRAC. IC50 DPPH value of all samples gave positively and significant
correlation with their EC50 CUPRAC. Conclusions: All extracts had antioxidant
activities. Phenolic compounds mainly contributed in antioxidant activities of
leaves aextract of S. jambos, S. samarangense, S. malaccense, dan S. aqueum by
DPPH and CUPRAC methods. DPPH and CUPRAC methods showed linear results
in antioxidant activities of all samples.
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