Optimation of Essential Oil Yield and Quality from Patchouli (Pogostemon cablin var. Patchoulina 2) Leaves Through Lignocellulose Biodegradation and Biodelignification of Distillation Feeds

• Main Author: Febrina Silaban, Naomi
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/40340
• Institution: Institut Teknologi Bandung

The aim of this study was to determine the influence of the biodelignification and degradation of Patchoulina 2 (Pogostemon cablin Beth.) lignocellulose structure’s leaves on the yield and patchouli oil content of the produced patchouli oil. The pretreatment was done in solid-state fermentation system and extraction of patchouli oil carried out by steam-distillation method. The variation of pretreatment was P-1 as a control of patchouli varieties of Sidikalang, P-2 as control of varieties patchoulina 2, P-3 had pretreatment of biodelignification in patchoulina 2 and P-4 had pretreatment of cellulose degradation in patchoulina 2. The biological agent used in the pretreatment of biodelignification was P.chrysosporium while the pretreatment of cellulose degradation was using T.viride. The result of fractionation of patchouli leave samples showed a decrease in lignocellulose components that occurred in P-3. Lignin component of P-3 was decreased by 22,73% due to lognolytic enzyme activity produced by P.chrysosporium. The reduction of lignin can decrease the value of cell wall retention so that the liquid content can diffuse effeiciently. This was confirmed by the increasing percentage of oil yield by 131,8% from 3,8% to 5,02% and patchoulol content by 112,7% from 58,38% to 65,82% from varieties of patchoulina 2 leaves as a control. The pretreatment of biodelignification using P.chrysosporium as biological agent showed the most potential pretreatment to improve the quality with acquisition of patchouli alcohol was 65.82% and quantity of patchouli oil variety patchoulina 2 was 5,02%.