EKSTRAK HERBA TIMI (THYMUS VULGARIS L.) SEBAGAI ANTIMIKROBA PADA SEDIAAN PASTA GIGI DAN PENGUJIAN AKTIVITASNYA SECARA IN VITRO TERHADAP STREPTOCOCCUS MUTANS

• Main Author: Sayyid Naufal, Muhammad
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/40378
• Institution: Institut Teknologi Bandung

Thyme has been known empirically and scientifically to have antimicrobial activities, especially against Streptococcus mutans, which is responsible for tooth decay in humans. However, its use as an active ingredient in formulation for dental health has not been found. The purpose of this research was to develop a toothpaste formulation containing thyme extract as an antimicroba and also as a solvent. Thyme extract was prepared by heating thyme in boiling aquadest, then steam was passed through a cooler, and the extract was collected in a closed container. The extraction process was carried out until the ratio extract to thyme is 3:1. Thymol contens in thyme extract was determined using TLC densitometer. The thymol content in thyme extract was 83,49 ppm. Thyme extract was tested for its antibacterial activity by determining the diameter size of growth inhibitory of Streptococcus mutans using disk diffusion method and the minimum inhibitory concentration (MIC) using microdilution method. Based on the results, thyme extract gave an activity of growth inhibition of Streptococcus mutans with a diameter 16,8±5,6 mm and MIC 256 ppm. The toothpaste formula consists of 30,1% kaolin, 15% glyserin, 7,5% propylen glykol, 2% titanium dioxide, 3% Na CMC, 2% sodium lauryl sulfat, 1% menthol, 0,2% sodium saccharin, 0,15% methyl paraben, 0,05% propyl paraben, and extract up to 100%. Toothpaste was tested for in vitro antimicrobial activity in the form of growth inhibitory of Streptococcus mutans colonies, which were quantified using a colony counter. Based on the results, toothpaste contains thyme extract gave 36,71% growth inhibiton of Streptococcus mutans.