ISOLASI SENYAWA FLAVONOID DARI DAUN KATUK (SAUROPUS ANDROGYNUS L. MERR.)
Katuk (Sauropus androgynus (L.) Merr.) is a plant that is widely used as traditional medicine. In Indonesia, katuk leaves are used as an ASI booster (laktagoga), treatment of boils, ulcers, zits and, fever. Based on research, one of secondary metabolites groups that found in katuk leaves is flavo...
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| Format: | Final Project |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/40379 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Katuk (Sauropus androgynus (L.) Merr.) is a plant that is widely used as traditional medicine. In
Indonesia, katuk leaves are used as an ASI booster (laktagoga), treatment of boils, ulcers, zits and,
fever. Based on research, one of secondary metabolites groups that found in katuk leaves is
flavonoids. Flavonoids have been widely used in the medical field because it have various activities
such as anti-inflammatory, antioxidant and antibacterial effects, so this study aims to isolate,
characterize, and identify flavonoid compounds that contained in katuk leaves. The crude drug of
katuk leaves were extracted by maceration method using methanol solvent. Methanol extract was
monitored using the thin layer chromatography (TLC) method with the citroboric spray reagent.
Chlorophyll separation is done by adding hot water to the methanol extract and filtering it out. The
filtrate obtained was fractionated by liquid-liquid extraction method using a solvent with multilevel
polarity namely n-hexane and ethyl acetate, so that three fractions were produced, namely n-hexane
fraction, ethyl acetate fraction, and water fraction. The three fractions were monitored by TLC
method. In the ethyl acetate fraction there were at least four flavonoid spots and in the water fraction
there were at least one flavonoid spot, whereas the n-hexane fraction was not observed of flavonoid
spot. Furthermore, the ethyl acetate fraction was sub-fractionated using preparative TLC method.
Subfraction which is containing flavonoid was purified by preparative TLC method, then purity was
tested by two-dimensional TLC and single development TLC with three different mobile phases.
Isolates were characterized by spectrophotodensitometry-TLC, ammonia vapor spray reagent, and
two-dimensional paper chromatography. Isolate is thought to be a compound of flavonol aglycone
with an OH group free at C3.
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