ISOLASI SENYAWA FLAVONOID DARI DAUN TRENGGULI (Cassia fistula L.)

• Main Author: Nurhayati, Nane
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/40411
• Institution: Institut Teknologi Bandung

Cassia fistula L. or trengguli is commonly found as an ornamental plant, pathways shade, and is often used as traditional medicine. The leaves in traditional medicines are used for laxative, fever, and skin remedy. Secondary metabolites have a role in the biological activity of a plant. One of many secondary metabolites found in trengguli leaves is flavonoid, which is responsible for antioxidant and antibacterial activity. Therefore, this study aims to isolate and identify the flavonoid compounds in trengguli leaves. In this study, the leaves were extracted with methanol by continuous extraction using Soxhlet apparatus, fractionated by liquid-liquid extraction using nhexane, ethyl acetate, and water. The fractions were monitored by thin layer chromatography (TLC) with silica gel GF254 as a stationary phase and ethyl acetate-n-hexane-methanol (8:1:1) as the mobile phase, and visualized using UV ?254 nm, UV ?366 nm, and citroboric spray reagent. Result showed that the largest flavonoid content was in the ethyl acetate fraction. The ethyl acetate fraction was sub-fractionated by preparative TLC using similar mobile phase and stationary phase of fraction monitoring. The subfraction was purified by preparative TLC with silica gel GF254 as a stationary phase and ethyl acetate-n-hexane-methanol (3:3:0.5) as the mobile phase, and visualized using UV ?254 nm, UV ?366 nm, and citroboric spray reagent. The purity was tested by single development TLC using three different mobile phases and two-dimensional TLC. The isolate was characterized and identified by spectrophotodensitometry, two dimensional paper chromatography, and ammonia spray reagent. It was concluded that the isolate was supposed to be an isoflavone compound without free 5-OH.