AKTIVITAS PENGHAMBATAN ENZIM XANTIN OKSIDASE DARI EKSTRAK, FRAKSI, DAN SUBFRAKSI EMPAT TANAMAN SUKU MORACEAE
Gout is a joint inflammatory disease caused by hyperuricemia or high levels of uric acid in the blood. This condition leads to the deposition of uric acid in the tissue which can induce inflammation. Some plants of Moraceae are traditionally used to treat the gout symptoms, therefore, further res...
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| Format: | Final Project |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/40412 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Gout is a joint inflammatory disease caused by hyperuricemia or high levels of uric acid in the blood.
This condition leads to the deposition of uric acid in the tissue which can induce inflammation.
Some plants of Moraceae are traditionally used to treat the gout symptoms, therefore, further
research is needed regarding the inhibitory activity of the xanthine oxidase enzyme which acts as a
catalyst in the formation of uric acid. This study was conducted in vitro by measuring the decrease
of uric acid absorbance using UV spectrophotometer at a wavelength of 290 nm. The decrease of
uric acid absorbance indicates the inhibitory activity of the xanthine oxidase enzyme which is
expressed in percent (%) inhibition. It was determined from the ethanol extract of breadfruit
(Artocarpus altilis (Parkinson) Fosberg.), jackfruit (Artocarpus heterophyllus Lamk.), chempedak
(Artocarpus integer (Thunb.) Merr.), and mulberry (Morus alba L.) leaves (at a concentration of
100µg/mL). Ethanol extract with the highest inhibitory activity was fractionated and the highest
activity fractions was determined for its IC50 (at a concentration of 100 µg/mL). The fraction was
then sub-fractionated and determined for the percent inhibition of each subfraction obtained (at a
concentration of 100µg/mL). Results showed that the highest inhibitory activity was found in the
breadfruit ethanol extract with percent inhibition of 12.81 ± 0.75 %. The ethyl acetate fraction from
breadfruit leaf ethanol extract had the highest activity with% inhibition of 17.16 ± 0.67 % and the
IC50 was 938 µg/mL, while IC50 of allopurinol was 1.24 µg/mL. The first spot from subfractionation
of ethyl acetate fraction gave the highest activity with percent inhibition of 22.09 ± 0.49%. Based
on the results, it was concluded that breadfruit leaves which traditionally used for gout treatment
have an inhibitory activity for the xanthine oxidase enzyme.
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