AKTIVITAS HIPNOTIK SEDATIF EKSTRAK ETANOL DAUN SELADA (LACTUCA SATIVA L.) TERHADAP MENCIT JANTAN GALUR SWISS WEBSTER

• Main Author: Nadiya Rahman, Zhafira
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/40520
• Institution: Institut Teknologi Bandung

Sleep disorder is a common clinical disorder, and one of the most frequently suffered is insomnia. Traditionally, Lettuce has been used for its nerve calming effect in the control of palpitations and to produce better sleep at night. This study aimed to determine the hypnotic-sedative effect of ethanolic extract of lettuce leaves in mice. Male Swiss Webster mice were used and divided into 5 groups comprised of control group, reference group, and test groups treated with ethanolic extract at doses of 100 mg/kg, 200 mg/kg, and 300 mg/kg. Behavioral observation was carried out one hour after oral administration of vehicle, reference drug or extract. Ketamine-induced sleeping was used to test hypnotic effect, while rotarod was used to test sedative property. Sleep latency, sleep duration, and sleep efficiency were observed for hypnotic activity. Meanwhile time spent in the rotatod and frequency of fall were assessed to d/etermine sedative effect. Results of the hypnotic test showed that the ethanol extract of lettuce leaves was unable to improve the efficiency of sleep compared to the control group. Meanwhile, the results of the sedative test showed that the ethanol extract of lettuce leaves at 100 mg/kg reduced the time spent on rotarod (55.25 ± 17.73 s) compared to the control group (427.25 ± 74.02 s), and increased the frequency of falls during the period of 180 seconds (1.75 ± 0.50) compared to control (no incident of fall). Overall, the data suggests that ethanol extract of lettuce leaves at a dose of 100 mg/kg bw has a sedative activity.