DETERMINATION OF MOTILITY AND CHARGE TYPE OF MENCIT SPERM (Mus musculus) THROUGH STUDY OF EXPOSURE ELECTRICAL AND MAGNETIC FIELDS

• Main Author: Trimayanti, Estri
• Format: Theses
• Language: Indonesia
• Subjects: Fisika
• Online Access: https://digilib.itb.ac.id/gdl/view/40766
• Institution: Institut Teknologi Bandung

Exposure of electric fields and magnetic fields affect sperm quality. Analysis of sperm quality is still one of the most important tools for evaluating a man's fertility. This sperm quality can affect fertilization. Sperm quality includes several aspects including motility or motion and viability. The purpose of this study was to determine the motility, viability, and type of charge of mice sperm (Mus muculus) through the study of exposure to electric and magnetic fields in ex vivo conditions. Observations were carried out in the time span (?t) for sperm without influence and that affected the electric field. Sperm of mice is diluted with a solution of Phosphate Buffered Saline (PBS) and then put into a hemasitometer placed between two iron plates connected to a DC voltage source. The distance between the two plates d = 7.0 cm and the magnitude of the voltage varied, namely ?V = 0 (control), V = 12.5 V, and V = 25.0 V. The magnitude of the magnetic field from the solenoid used is B = 4.7 × 10-4 T. Sperm observed using a fluorescence microscope then recorded using NIS Elements software. Furthermore, sperm movement is analyzed using Tracker software. Sperm without electric field (?V = 0) has a viability of 60 minutes longer than sperm that is affected by the electric field (?V = 12.5V and ?V = 25.0V) which has a viability of 30 minutes. Sperm moves randomly almost in all directions if without the influence of the electric field, but sperm tends to move to the positive plate if it is under the influence of the electric field. Normal sperm quality is characterized as having zigzagging sperm that is fast and 25%. From the results of this study the results showed that fast zigzag motion had a velocity of v > 37 ?m/s. The maximum rate decreases with increasing observation time. Whereas in the influence of the magnetic field the sperm moves in a clockwise direction. Based on the direction of sperm movement it can be seen that sperm are negatively charged.