Nutrition Optimization and Characterization of Extra Polymeric Substance Bacillus licheniformis DS1 for Microbial Enhanced Oil Recovery (MEOR) Application

• Main Author: Nur Afifah, Lulu
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/41270
• Institution: Institut Teknologi Bandung

Microbial enhanced oil recovery (MEOR) is a method that can used to enhance tertiary oil recovery. Bacteria in MEOR can produced several metabolites including biopolimer. Bacillus licheniformis DS1 isolated from Sumatera Oilfield in Indonesia known to have ability to biopolimers, but its biopolymers has not yet characterized. The main problem producing biopolimer from bacteria is high cost of fermentation and carbon source. The aims of this research were to optimize the nutrition for B. licheniformis DS1 for biopolimers production, characterize biopolimer, and determine its ability to enhance oil recovery. Before nutrition optimization, concentration range for N and P source was carried. Nutrition optimization was carried using Central Composite Design Response Surface Methodology using software Design Expert (CCD RSM) with molasse as a carbon source. Optimum concentration was used to made growth and production curve of biopolimers. Functional group of biopolimers determined by FTIR. Biopolimers were characterized the stability at differet salinity and temperature, the rheology test, carbohydrate test by Phenol Suphuric Assay, protein test by Bradford Assay, flocculation activity test using Kaolin Clay Assay. Oil recovery by biopolimer was tested by Sandpack Column Assay (SCA). Optimal nutrition obtained from CCD RSM was molasse 6.4%, KH2PO4 0.5%, and NH4Cl 0.32%. Biopolimers showed having alkane, alkene, alcohol and eter functional group. Biopolimers known as a pseudoplastic fluid with n = 0.152 and viscosity 21 cP. B. licheniformis DS1 biopolimers have 121 ppm carbohydrate, 8.9 ppm protein and have 97.7% flocculating activity. Secondary oil recovery from SCA from the highest AOR were with biopolimers extract 47.7%, bacteria injection 35.8% and supernatant injection 26.67%.