Determination of Phenol And Its Derivatives And Polycyclic Aromatic Hidrokarbon In Layang Fish (Decapterus Macrosoma) From Marunda Beach By HPLC Method
Organic pollutants such as oil, grease, and phenols are harmful pollutants, especially on the biota that can cause physiological disorders. In addition, the compounds polycyclic aromatic hydrocarbons (PAH) are found in the waters, although in very small quantities. Some of these compounds are carcin...
Saved in:
| Main Author: | |
|---|---|
| Format: | Final Project |
| Language: | Indonesia |
| Subjects: | |
| Online Access: | https://digilib.itb.ac.id/gdl/view/41349 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Organic pollutants such as oil, grease, and phenols are harmful pollutants, especially on the biota that can cause physiological disorders. In addition, the compounds polycyclic aromatic hydrocarbons (PAH) are found in the waters, although in very small quantities. Some of these compounds are carcinogens. Therefore, this research analyzed the phenol derivative and polycyclic aromatic hydrocarbons (PAH) compounds in fish samples. Fish can be regarded as indicators of pollution because they are showing a reaction to the physical changes to their water and pollutant compounds that are dissolved in a certain concentration limits. For this study, the use of layang fish (Decapterus Macrosoma) from Marunda Beach area because the area is a commercial and industrial area that is predicted to damage the ecological balance. To analyze the phenol derivative used method of freeze dry first. Subsequently, soxhlet all samples using dichloromethane: methanol (1:1), after that, the samples were analyzed using high performance liquid chromatography (HPLC) with reversed phase C18 column (column length 25 cm), UV detector, methanol: water (55:45) as eluent, and the water rate of 0.8 mL / min. Meanwhile, in order to analyze the presence of polycyclic aromatic hydrocarbons (PAH), first, saponified samples with KOH solution and refluxing, and then extract using n-hexane. Aromatic compounds separated by column chromatography using alumina column:silica gel (1:1) with dichloromethane as eluent. The results of the process of this column were analyzed using high performance liquid chromatography (HPLC) with reversed phase C18 column (length 25 cm), UV detector, methanol:water (85:15) as eluent, and the rate of water 1 mL/min. For summary, the concentration of aminophenol compound and naphthalene compound are 4.76 ppm and 2.289 ppm respectively. |
|---|