BETA CAROTENE SUPPLEMENTATION FROM TONGKAT LANGIT BANANA TO ACCELERATE HAIR CELLS AND CAUDAL FIN REGENERATION IN ZEBRAFISH

• Main Author: Mulyani Winata, Grace
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/41417
• Institution: Institut Teknologi Bandung

Tongkat langit banana (Musa troglodytarum), a functional food from Indonesia, has a higher beta carotene content than other bananas. In this study, M. troglodytarum from Mount Galunggung, West Java was used. Beta carotene as an antioxidant has been studied to accelerate tissue regeneration and increase gene expression activity of enzymes related to defense against oxidative stress states. In addition, astaxanthin (one of carotenoid group) play a role as an otoprotectant. Expression of genes related to defense against ROS can be used as biomarkers of oxidative stress, such as prdx1 and hsp70 protein. Hsp70 can also be used as a biomarker for tissue regeneration. However, no studies have been conducted to study beta carotene as otoprotectant on hair cells. Then, there are not many researches that study the effects of beta carotene intake on gene expression related to oxidative stress and the rate of regeneration. This research aims to study the effects of beta carotene from M. troglodytarum on protection of hair cells, rate of tissue regeneration, and expression of gene biomarkers related to defense against oxidative stress in zebrafish. In this study, there are 2 kind of 15 ?M ?-carotene treatment, preventive and curative, that given to 5 dpf zebrafish larvae. Preventive treatment aims to study the effects of ?-carotene as otoprotectant. This study used 2 methods to increase oxidative stress, neomycin intake which remove hair cells and caudal fin amputation. Furthermore, regeneration of hair cells was observed at 24 and 48 hour after neomycin intake. Whereas caudal fin regeneration was observed at 24, 48, 72 and 96 hour post amputation. Expression of prdx1 and hsp70 were used as oxidative stress biomarkers and were analyzed using qPCR method. ?-carotene intake, preventive and curative, can accelerate tissue regeneration in 24 hour compared to control (p<0,05). However, ?-carotene did not act as otoprotective and did not increase the expression of genes that maintain cells from oxidative stress conditions.