Preparation and Characterization of Pectin/CMC/MCC Composite as Second Degree Burn Wound Dressing on Wistar Rat (Rattus norvegicus domestica) Model

• Main Author: Alfath, Muhammad
• Format: Final Project
• Language: Indonesia
• Subjects: Kimia
• Online Access: https://digilib.itb.ac.id/gdl/view/42067
• Institution: Institut Teknologi Bandung

Porous scaffolds made of biopolymers are the main recent topic in tissue engineering for living organisms. Biopolymers are choosen because they are cheap, widely available in nature, non-toxic, and non-allergic. [n this research, a novel material was developed in the fonn of composites made of pectin, carboxymethyl cellulose (CMC) and microcrystalline cellulose (MCC) using lyophilization technique. The resulting composites were characterized and applied in healing second-degree bums in the Wistar rat model. The most optimum composite optimization consisted of pectin 0.3% (w/v), CMC 0.12% (w/v) and MCC 0.03% (w/v) having the ideal pore size (d = 30-300 un) for the growth of fibroblasts in the process of wound closure. Addition ofMCC to the composites can reduce the rate of in vitro degradation of the composites in a phosphate buffer system of pH 7.4 and temperature of 37 oc. The addition ofMCC to the composites was also able to significantly increase the thermal resistance of the composites compared to the ones without MCC. In vivo studies conducted on Wistar rats showed significant different results (p < 0.05) between the treated group and the negative control group. On the 2 P' day, the remaining wound size in the treated group was 16.38 ± I 0.04% and the remaining wound size negative control group was 43.59 ± 19.10%. These results indicated that the pectin/CMC/MCC composite material has the potential to be used as a second-degree bum wound dressing.