Bioconversion Study of Lauric Acid Became Dodecanedioic Acid Using Candida tropicalis by Adding Acrylic Acid as Inhibitor

• Main Author: Febrina, Yeti
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/42072
• Institution: Institut Teknologi Bandung

Dodecandioic acid (3DA) is a dicarboxylic acid which is often used as a precursor for industrial products. 3DA production in this study uses the Candida tropicalis as biocatalyst by using fatty acids as substrates. The fatty acid substrate is lauric acid as a renewable raw material derived form coconut oil. In this study, the effect of using acrylic acid as an inhibitor to increase 3DA production by Candida tropicalis wild-type yeast will be studied. The fermentation process is carried out in an erlenmeyer flask with a working volume of 300 ml. Variations made are variations in the time of addition of acrylic acid (first and fourth day) and variations in the concentration of acrylic acid (0 g/L; 0,5 g/L; 1,0 g/L; and 2 g/L). Addition of acrylic acid to the fermentation solution can reduce the rate of cell growth and the rate of consumption of lauric acid in the fermentation solution. This can be seen in the decrease in cell concentration in the solution in the range of 15-20% and stable during the fermentation process. The addition of acrylic acid with a concentration of 0,5 g/L and 1,0 g/L did not have a significant effect on increasing 3DA production in the fermentation solution, while the concentration of 2,0 g/L acrylic acid had a significant effect. This can be seen in the maximum 3DA concentration obtained at the addition of 2,0 g/L acrylic acid on day 0 and 4th day that is equal to 0,33 g/L and 0,56 g/L. This study shows the potential effect of acrylic acid at concentrations greater than 2,0 g /L to increase 3DA production. In addition, on the 4th day is a better time to add acrylic acid because at that time the highest number of cells is reached in the system by adding lauric acid as a substrate.