Making of Particle for High Performance Liquid Chromatography Colmnn from 3-APTMS Modified Silica for Sugar Separation

• Main Author: Nuzula Ramdhiny, Masytha
• Format: Final Project
• Language: Indonesia
• Subjects: Kimia
• Online Access: https://digilib.itb.ac.id/gdl/view/42154
• Institution: Institut Teknologi Bandung

High Performance Liquid Chromatography (HPLC) is the most suited analytical method for sugar analysis based on its accuracy, simplicity on sample preparation, and analysis duration compared to any other methods. Silica amine (Si-NH2 ) column is commonly used in HPLC for sugar analysis, however, it is considered expensive for a column. In this research, a Si-NH2 column has been made with silica particles with the size of 30-60 micron which is being modified with 2% and 20% (3-Aminopropyl)trimethoxysilane (3-APTMS). The first step in this experiment begin with activating the silica particles using piranha solution made up of H so. an d 1-1 2 0 30% (3: I). The activated silica is then reacted with 3-APTMS for 24 hours in order to obtain Si-NJ-12 particles, and some of the Si-NH 2 particles go through deactivating process with triethylamine. Every step is followed by characterization using FT-IR and at the end SEM analysis is done. According to FT-IR data, Si-NJ-12 particles are successfully made due to new peaks fanned on the spectrum such as peaks from Si-C (800-950 cm- 1 ), C-N (1000-1350 cm- 1 ), dan N-1-1 ( 1550-1650 cm-1 ). Afterwards, Si-NJ-1 particles are loaded to HPLC column with size of 4.6 mm x I 00 mm. Column is being tested upon glucose, maltose, and a mixture of both which are made by three different solvents similar to the eluents used. Eluents used involves water, aceton itrile, and water-acetonitrile (50:50 ). Based on the experiment, Si-NJ-12 made with 2% 3-APTMS (Si -NJ-12 2%) with eluent of water-acetonitrile (50:50 ) gives the best perfonnance where glucose and maltose sample peaks are formed on different retention time with significant peak shapes. According to SEM data obtained, activat ion and attaching primary am ine on si lica surface have been done successfu ll y.