Antioxidant Activity of ,8-Carotene and Its Derivative from Cyclotella striata strain TBI

• Main Author: Rahayu Ningtyas, Zeni
• Format: Final Project
• Language: Indonesia
• Subjects: Kimia
• Online Access: https://digilib.itb.ac.id/gdl/view/42214
• Institution: Institut Teknologi Bandung

C'yclotella stnala strain TBI is a tropical marine diatom producing ft-carotene that posseses anticancer, antiinflammatory, and antioxidant activities. The purpose of this research was to determine the antioxidant activities of P-carotene obtained from C. slrrala strain TBI and its derivative compound from metathesis reaction product. The methods included production of C. :•)tnata strain TBI biomass. isolation and purification of /]-carotene from the biomass. transformation of P-carotene into its derivative compound through metathesis reactions, and determination antioxidant activities of fJ-carotene and its derivative. The biomass productivity of C. Slrlala strain TBI cultivated in a 1500-L photobioreactor with an initial concentration of 500.000 cells/mL was in a range of 0.274-0.407 g cell/Ud. P-Carotene was identified by TLC at an R1 value of 0.98. and maximum absorption at wavelengths of 450 and 478 nm. The structure of ft-carotene dete1mined by 1 H- MR 500 MHz observed at chemical shifts of 0.81 ppm (methyl end, -CH1); 1.05-1.39 ppm (CH::CHrCH2); 1.44 ppm (C-CH ,); 1.50--191 ppm (-C(CH3 h): 1.97 ppm (-CH;;); and 6.15-D.98 ppm (conjugated olefin. -CH=CH-CH-). The structure was also confirmed by 11C-NMR 125 MHz at chemical shifts of 14.38 ppm (-CH1): 22.81 ppm (C-CH -C): 27.09 ppm (C-CH1): 29.70 ppm (-CH 1); 31.93 ppm (C(CH1)2): 38.38 ppm (-CH;;CH-); 129.97, 136.44 ppm (CH­ CH=CH); and 125.50. 126.64. 130.83, 132.41. and 137. 77 ppm (CH=C(CH,) -CH). The antioxidant activity of ft-carotene obtained from C. stnata strain TBI was 0. 23. Meanwhile, the antioxidant activity of fi-carotene de1ivative compound was 0.39. So, ft-carotene derivative compound from metathesis reaction product has an antioxidant activity 1.7 times higher.