Susceptibility Status of Aedes aegypti Larvae to Temephos in Sekejati, Bandung City

• Main Author: Zharifa Khansa, Dzakira
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/42435
• Institution: Institut Teknologi Bandung

Dengue hemorrhagic fever (DHF) is an endemic disease in Indonesia that is transmitted to humans through mosquito bites, especially the Aedes aegypti mosquito species, which has been infected with the dengue virus. Ways to control mosquitoes as dengue vectors includes using temephos larvicide. However, a long-term use of larvicides can cause resistance to larvae as there has been reported in many locations in Indonesia. However, previous research determined the level of resistance to temephos in a large area (city) so that it did not provide information at the small scale level (kelurahan), especially in areas with high population density. Based on this information, this study aims to determine the susceptibility status of Aedes aegypti larvae against temephos larvicide in Sekejati, Bandung. In this study, Aedes aegypti larvae were obtained from 4 different RWs in Sekajati using ovitrap. The larvae used for resistance testing were 3rd and 4th instar larvae. Resistance tests were carried out based on WHO standard procedures with 5 different temephos doses of 0.2 ppm, 0.4 ppm, 0.6 ppm, 0.8 ppm and 1 ppm. Larval mortality was calculated after 24 hours of exposure and analyzed using Kruskal-Wallis test and LC50 from each RW was calculated using a probit analysis and then the value of resistance ratio (RR) in each RW were obtained. The results showed that RR values varied between 0.26-2.67 in the four RWs. The conclusion from this study is the larvae of Aedes aegypti originating from Sekejati Village, Bandung City, West Java in general are still vulnerable to temephos.