PERFORMA SISTEM ZERO WATER DISCHARGE DENGAN PENAMBAHAN KONSORSIUM HALOMONAS AQUAMARINA, HALOMONAS ALKALIPHILA, BACILLUS CEREUS, CHAETOCEROS SP., DAN BAKTERI NITRIFIKASI PADA PADAT TEBAR UDANG PUTIH (LITOPENNAEUS VANNAMEI) BERBEDA

• Main Author: Ciamorien, Yoghi
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/43021
• Institution: Institut Teknologi Bandung

Zero Water Discharge (ZWD) is one of the closed system shrimp culture technologies that can allow stable production by improving water quality in shrimp farming. Based on previous studies, the ZWD system with the addition of probiotics Halomonas aquamarina, H. alkaliphila, Bacillus cereus, microalgae Chaetoceros sp., and nitrifying bacteria can increase the growth and production of white shrimp (Litopennaeus vannamei) culture. However, the optimum stocking density for this system has not been determined. Stocking density is one of the important factors in cultivation to achieve economical effectiveness. This study aims to determine the optimum stocking density in shrimp aquaculture using ZWD system with the addition of H. aquamarina, H. alkaliphila, B. cereus, Chaetoceros sp., and nitrifying bacteria. This study tested three different stocking densities treatments; (1) 300 PL/m3 (A); (2) 400 PL/m3 (B); (3) 500 PL/m3 (C) on a semi-mass scale with total volume 300 L and cultured for 77 days. Water quality, biological and microbiological parameter are measured to determine the optimum stocking density. The results showed that treatment A had the best performance based on survival parameter (81.00±9.35%), feed conversion ratio (1.39±0.167), and productivity (2.10±0.25 kg /m3) and significantly different with other treatments (P <0.05). Water quality parameters showed that all treatments had similar NH4+, NO3-, and PO43- concentrations, while the lowest NO2- concentration (0-6.67 ppm) was found in treatment A and was significantly different from other treatments starting from 6th week to the 11th week (P <0.05). Treatment A also has a total of Vibrio sp. water (102–104 CFU/mL) and shrimp (103-105 CFU/gram shrimp) are lower than other treatments. Based on community-level physiological profiling results, all treatments had microbial community that mostly utilize amino acids and polymers as main carbon sources. Based on this research, the optimum stocking density in the ZWD system with the addition of H. aquamarina, H. alkaliphila, B. cereus, Chaetoceros sp. and nitrifying bacteria is 300 PL/m3.